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Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie [M. H., C. T., H. P., P. H.], Universität Karlsruhe, Institut für Genetik [W. R., P. H., U. G.], D-7500 Karlsruhe 1, and Deutsches Krebsforschungszentrum Heidelberg, Institut für Radiologie und Pathophysiologie, Im Neuenheimer Feld 280 [M. Z.], D-6900 Heidelberg 1, Federal Republic of Germany
One of several splice variants of CD44 expressed in metastasizing cell lines of rat tumors has been shown to confer metastatic potential to the non-metastatic variant of a rat pancreatic carcinoma line (U. Günthert et al., Cell, 65: 13–24, 1991). The variant-specific rat CD44 sequences were used to detect RNA expression in human cell lines: in carcinoma lines from lung, breast and colon; and in keratinocyte lines.
By polymerase chain reaction amplification, complementary DNAs encoding human homologues were isolated and sequenced. The largest splice variant has been found in a large cell lung carcinoma line and in keratinocyte cell lines. It carries at least 5 additional domains (exons) encoding a total of 338 amino acids in the membrane-proximal extracellular region of the standard CD44. Various alternative splice products have been detected in other human tumor cell lines. The distribution of CD44 splice variants is consistent with the speculation that they fulfill functions in only a few restricted differentiation pathways and that in tumor cells these pathways have been reactivated.
1 This investigation was supported by Deutsche Forschungsgemeinschaft Grants Gü 249/1-1 to U. G. and He 551/6-1 to U. G., P. H., H. P., and M. Z.
Received 4/ 2/91. Accepted 7/16/91.
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