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Structure and Expression of the ß-Platelet-derived Growth Factor Receptor Gene in Human Tumor Cell Lines¹

Division of Oncology, Children's Hospital of Philadelphia, and the Department of Pediatrics, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Southern digests of DNA from 15 human connective tissue tumor-derived cell lines (and cell strains) and from peripheral blood lymphocytes from normal volunteers were prepared to compare structure of the ß-platelet-derived growth factor receptor gene in normal and tumor-derived cells. The tumor-derived samples had no alterations in gene structure, nor was gene amplification evident. Two restriction fragment length polymorphisms were detected within the ß-receptor gene. Expression of the - and ß-platelet-derived growth factor receptor was quantified using Northern blots. Expression was not tumor-type specific. For example, one rhabdomyosarcoma cell line expressed only the -receptor, whereas two others expressed the ß. In contrast, a human fibroblast cell strain expressed both receptor types. Alterations in receptor expression may be a result of the tumorigenic process.

¹ This work was supported by NIH Grant CA47983. C.D.S. and R.B.W. were supported in part by "The Eagles Fly for Leukemia" campaign.

² Present address: Department of Pediatrics, Duke University Medical Center, Durham, NC 27710.

³ Present address: Creighton Cancer Center, Creighton University, Omaha, NE 68178.

⁴ Present address: Wistar Institute, Philadelphia, PA 19104.

⁵ To whom requests for reprints should be addressed, at Children's Hospital of Philadelphia, 34th and Civic Center Boulevard, Philadelphia, PA 19104.

Received 6/12/91. Accepted 8/ 5/91.