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Human Malignant Histiocytosis CD30+ DEL Cell Line Differentiates into Macrophage-like Cells When Treated with a Phorbol Diester¹

Institut National de la Santé et de la Recherche Médicale U 90 [J. G.] and Groupe de Pathologie Pédiatrique [S. B., C. N.], Hôptial Necker Enfants Malades, 149, rue de Sèvres, 75743 Paris Cedex 15, France

The histiocytic of lymphoid origin of human malignant histiocytosis is currently a subject of debate. The aim of this study was to investigate the in vitro effects of 12-O-tetradecanoylphorbol-13-acetate used as a differentiation inducer on the CD30, t(5;6) (q35;p21) DEL cell line, taken to be a reliable representative of the human malignant histiocytosis cell line.

Treatment of DEL cells with 33 nM 12-O-tetradecanoylphorbol-13-acetate for 6–24 h resulted in cell surface attachment (up to 80%), decrease in dividing ability, enhancement of nitro blue tetrazolium reducing capacity (from 8 to 42%), occurrence of a limited immunodependent phagocytosis, and transient increase in expression of tumor necrosis factor gene and in production of tumor necrosis factor protein, whereas tumor necrosis factor ß remained undetectable. From these data, we can conclude that the malignant histiocytosis DEL cell line is not of lymphoid origin but stems from a myelomonocyte lineage.

¹ This research was supported by grants from the Association pour la Recherche sur le Cancer and the Conseil Scientifique de l'Université Paris V.

² To whom reprint requests should be addressed, at 27 rue Gazan, 75014 Paris, France.

Received 3/29/91. Accepted 8/ 1/91.