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Rearrangements on Chromosome 11q23 in Hematopoietic Tumor-associated t(11;14) and t(11;19) Translocations¹

Laboratories of Chemotherapy, Immunology and Ultrastructure Research, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464 [Y. A., Ma. S., T. T., K. R. U., R. U.]; Department of Pediatrics, Keio University School of Medicine, Shinjuku-ku, Tokyo 160 [Mi. S.]; and Department of Pediatrics, Yamanashi Medical College, Nakakoma-gun, Yamanashi 409-38 [S. N.], Japan

We previously demonstrated that the breakpoint of t(11;14)(q23;q32) in the RC-K8 B cell lymphoma cell line lies between CD3 and THY1/ETS1 on chromosome 11q23, and we cloned this region and named it the rck locus. Pulsed-field gel electrophoresis showed that the rck probe B (distal to the breakpoint) and the porphobilinogen deaminase (PBGD) probe detect the same germ line band and also the same rearranged band when DNA from RC-K8 cells was digested with NotI enzyme. Furthermore, Southern blot analysis with somatic cell hybrids showed that the PBGD gene moved to the 14q+chromosome, which confirmed PBGD to be more distal to the centromere than the rck locus. These data allowed us to construct the following order of genes: 11 cen-q23-CD3-rck-PBGD-THY1/ETS1. In this study, three infantile leukemia cell lines with t(11;19) (q23;p13) translocation were also analyzed by pulsed-field gel electrophoresis. CD3D probe detected the rearranged bands in DNA from two of them after digestion with NotI and SacII enzymes, demonstrating that the breakpoints of both cell lines were estimated to be within 360 kilobases of CD3D.

¹ This work was supported in part by a Grant-in-Aid for the Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health and Welfare; a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan; and a grant from the Cancer Research Institute, Inc., New York.

² To whom requests for reprints should be addressed.

Received 10/22/91. Accepted 10/29/91.

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