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Effects of Type of Dietary Fat on Phorbol Ester-elicited Tumor Promotion and Other Events in Mouse Skin¹

University of Texas M. D. Anderson Cancer Center, Science Park - Research Division, Smithville, Texas 78957 [J. L., M. L. L., T. J. S., S. M. J.]; University of Texas at Austin, Division of Nutrition, Austin, Texas 78712 [M. L., M. A. B.]; and Best Foods, Inc., Research and Engineering Center, Union, New Jersey 07083 [D. B.]

Based on the biological activity of arachidonic acid metabolites, we hypothesized that alterations in the consumption of linoleic acid, the precursor to arachidonic acid, would result in a modification in tumor development when fed during the tumor promotion stage of the mouse skin initiation-promotion model. The effects of seven different levels of dietary linoleic acid (LA), supplied as corn oil in a 15% fat diet, on the incidence and rate of papilloma and carcinoma development were determined. SENCAR mice were placed on one of the experimental diets, containing 1.0, 3.6, 6.0, 7.9, 9.9, 12.5, or 15.0% corn oil, 1 week after initiation with 10 nmol of 7,12-dimethylbenz(a)anthracene and 3 weeks prior to the start of twice weekly promotion with 1 µg 12-O-tetradecanoylphorbol-13-acetate (TPA). At 15 weeks of TPA treatment there were significant differences in papilloma number among diet groups, such that an inverse correlation (r = 0.92) was observed between tumor number and level of corn oil; the lowest corn oil diet group had an average of 11.7 tumors/mouse, while the highest corn oil group had 5.4 tumors/mouse. However, there was little difference in tumor incidence among diet groups. A general relationship between diet and carcinoma incidence was also found, such that the highest corn oil diet group had the lowest carcinoma incidence. In an experiment performed with DBA/2 mice, the average number of papillomas/mouse at 17 weeks was 4.5 (1.0% corn oil), 5.6 (7.9% corn oil), and 2.3 (15.0% corn oil). Papilloma incidence was also affected by diet, with a 79% incidence for the 15.0% corn oil and an incidence of 93% for the 1.0% corn oil group. Analyses of the fatty acid composition of epidermal phospholipids in mice fed the experimental diets reflected the dietary LA levels, in that an accumulation of phospholipid LA, accompanied by an overall decrease in arachidonic acid, occurred with increasing dietary corn oil. In spite of the high membrane levels of LA, no measurable amount of epidermal conjugated dienes of LA could be detected. Epidermal prostaglandin E₂ levels in acetone-treated mice were similar for all diet groups (approximately 3 pg/µg DNA). However, 6 h after topical application with 4 µg of TPA, prostaglandin E₂ levels were elevated 5- to 10-fold; an inverse correlation (P < 0.05) was seen with increasing dietary LA, although the concordance with decreased phospholipid arachidonic acid was not strong. The extent of the hyperplastic response of the epidermis to 1 µg TPA showed no correlation with increasing dietary LA, in that no differences were seen among the diet groups. These studies indicate that increasing dietary intake of corn oil (or decreasing saturated fat) in the tumor promotion stage of multistage carcinogenesis in mouse skin results in significant suppression of tumor development. The observations from this study also suggest that, while such diets may offer protection against tumor development by reducing prostaglandin synthesis, it is probable that other mechanisms are operative as well.

¹ This work was supported by NIH Grant CA 46886 and by Best Foods, Inc.

² To whom requests for reprints should be addressed.

Received 7/24/90. Accepted 11/15/90.

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