This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sugimoto, Y. Articles by Tsuruo, T. Search for Related Content PubMed PubMed Citation Articles by Sugimoto, Y. Articles by Tsuruo, T.

Suppression of Experimental Lung Colonization of a Metastatic Variant of Murine Colon Adenocarcinoma 26 by a Monoclonal Antibody 8F11 Inhibiting Tumor Cell-induced Platelet Aggregation¹

Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Kami-Ikebukuro, Toshima-ku, Tokyo 170 [Y. S., M. W., T. O., S. S., T. T.], and Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113 [T. I., T. T.], Japan

We have previously established and characterized two monoclonal antibodies, 8F11 and 20A11, that recognize an M_r 44,000 membrane glycoprotein of metastatic murine colon 26 cells. Both monoclonal antibodies inhibit platelet aggregation induced by the tumor cells in vitro. In this report, the inhibitory effect of 8F11 on lung colonization of i.v.-inoculated tumor cells was examined. The i.v. administration of 8F11 suppressed lung colonization of NL-17, a highly metastatic variant of colon 26. Inhibition of NL-17 lung colonization by 8F11 was dose dependent with a maximum of 80% inhibition at a dose of 800 µg 8F11/mouse. 8F11 did not inhibit metastases at doses lower than 100 µg/mouse. Inhibition of pulmonary metastases by 8F11 was greatest when the antibody was administered 2 h before tumor inoculation. The effect was diminished when the antibody was given 2 h after tumor inoculation. The pulmonary retention of i.v.-inoculated radiolabeled NL-17 cells was decreased by 8F11. F(ab')₂ fragments of 8F11 also effectively inhibited lung colonization by NL-17 cells, suggesting that mechanisms unrelated to immune-mediated destruction are involved. These results indicate that the monoclonal antibody 8F11 suppresses the lung colonization of NL-17 cells by interfering with the initial arrest of tumor cells in the lung vasculature through the inhibition of tumor cell-platelet interaction.

¹ This work was supported by Grants for Cancer Research from the Ministry of Education, Science and Culture, Japan, and from the Vehicle Racing Commemorative Foundation.

² To whom requests for reprints should be addressed.

Received 4/11/90. Accepted 11/20/90.

This article has been cited by other articles:

				K. Suzuki-Inoue, Y. Kato, O. Inoue, M. K. Kaneko, K. Mishima, Y. Yatomi, Y. Yamazaki, H. Narimatsu, and Y. Ozaki Involvement of the Snake Toxin Receptor CLEC-2, in Podoplanin-mediated Platelet Activation, by Cancer Cells J. Biol. Chem., September 7, 2007; 282(36): 25993 - 26001. [Abstract] [Full Text] [PDF]

				A. Kunita, T. G. Kashima, Y. Morishita, M. Fukayama, Y. Kato, T. Tsuruo, and N. Fujita The Platelet Aggregation-Inducing Factor Aggrus/Podoplanin Promotes Pulmonary Metastasis Am. J. Pathol., April 1, 2007; 170(4): 1337 - 1347. [Abstract] [Full Text] [PDF]

				Y. Kato, N. Fujita, A. Kunita, S. Sato, M. Kaneko, M. Osawa, and T. Tsuruo Molecular Identification of Aggrus/T1{alpha} as a Platelet Aggregation-inducing Factor Expressed in Colorectal Tumors J. Biol. Chem., December 19, 2003; 278(51): 51599 - 51605. [Abstract] [Full Text] [PDF]

				L. Oleksowicz, N. Bhagwati, and M. DeLeon-Fernandez Deficient Activity of von Willebrand's Factor-cleaving Protease in Patients with Disseminated Malignancies Cancer Res., May 1, 1999; 59(9): 2244 - 2250. [Abstract] [Full Text] [PDF]