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Interstitial Chromosomal Deletion within 4q11–q13 in a Human Hepatoma Cell Line¹

Department of Medical Biochemistry, University of Calgary, Calgary, Alberta T2N 4N1, Canada [Y. U., K. W., T. T.]; Department of Pathology and Laboratory Medicine, University of Alberta, Edmonton, Alberta T6G 2R7, Canada [C. C. L.]; and Department of Pathology, Cancer Institute, Toshima-ku, Tokyo 170, Japan [O. H.]

Southern blot analysis revealed the presence of an aberrant albumin gene as well as a normal one in a human hepatoma cell line, HuH-7. A genomic sequence carrying this altered gene was isolated and characterized. This clone contains a 3-kbp 3' segment of the albumin gene linked to a non-albumin sequence at intron 11. The non-albumin sequence is assigned to chromosome 4q12–q13 by in situ hybridization. This indicates an interstitial deletion of a chromosomal segment within 4q11–q13 because the albumin gene is mapped there. The truncated albumin gene is detected in an early passage of HuH-7 cells and has been maintained stably in cell culture.

¹ This work was supported by the National Cancer Institute of Canada, the Medical Research Council of Canada, and Japan Immuno Research Laboratory, Inc.

² Present address: Department of Dermatology, University of Tokushima, Tokushima 770, Japan.

³ Present address: Department of Biochemical Engineering and Science. Kyushu Institute of Technology, lizuka, Fukuoka 820, Japan.

⁴ To whom requests for reprints should be addressed, at Department of Medical Biochemistry, University of Calgary, Calgary, Alberta T2N 4N1, Canada.

Received 10/ 4/90. Accepted 12/19/90.

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