| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Laboratory of Cell Biology [S. S. C., M. M. G.] and Laboratory of Molecular Biology [L. J. G.], National Cancer Institute, NIH, Bethesda, Maryland 20892
It has been proposed that proteases secreted by cancer cells facilitate tumor invasion and metastasis by degrading the components of extracellular membranes. The lysosomal cysteine protease cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells in culture. The secreted protease is potent in degrading collagen, laminin, elastin, and other structural proteins of basement membranes. To determine whether human cancers synthesize cathepsin L, the expression of cathepsin L in approximately 100 human tumor samples was determined by quantitative RNA slot blot analysis using a specific human cathepsin L complementary DNA probe. Results of the present study suggest that cancers in general express higher levels of cathepsin L than do normal tissues. Kidney and testicular tumors expressed the highest levels of cathepsin L; non-small cell carcinomas of the lung expressed the next highest levels; and most cancers of the breast, ovary, colon, adrenal, bladder, prostate, and thyroid expressed elevated levels as well. Cathepsin L may prove useful as a diagnostic or prognostic marker of human malignancy.
1 To whom requests for reprints should be addressed, at Laboratory of Cell Biology, National Cancer Institute, NIH, Building 37, Room 1B22, Bethesda, MD 20892.
Received 10/ 1/90. Accepted 12/14/90.
This article has been cited by other articles:
|
|
 |
|
  F. Boudreau, C. R. Lussier, S. Mongrain, M. Darsigny, J. L. Drouin, G. Doyon, E. R. Suh, J.-F. Beaulieu, N. Rivard, and N. Perreault Loss of cathepsin L activity promotes claudin-1 overexpression and intestinal neoplasia FASEB J, December 1, 2007; 21(14): 3853 - 3865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Goulet, L. Sansregret, L. Leduy, M. Bogyo, E. Weber, S. S. Chauhan, and A. Nepveu Increased Expression and Activity of Nuclear Cathepsin L in Cancer Cells Suggests a Novel Mechanism of Cell Transformation Mol. Cancer Res., September 1, 2007; 5(9): 899 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ravanko, K. Jarvinen, J. Helin, N. Kalkkinen, and E. Holtta Cysteine Cathepsins Are Central Contributors of Invasion by Cultured Adenosylmethionine Decarboxylase-Transformed Rodent Fibroblasts Cancer Res., December 15, 2004; 64(24): 8831 - 8838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rousselet, L. Mills, D. Jean, C. Tellez, M. Bar-Eli, and R. Frade Inhibition of Tumorigenicity and Metastasis of Human Melanoma Cells by Anti-Cathepsin L Single Chain Variable Fragment Cancer Res., January 1, 2004; 64(1): 146 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Nishimura, H. Naruishi, K. Naruishi, T. Yamada, J. Sasaki, C. Peters, Y. Uchiyama, and Y. Murayama Cathepsin-L, a Key Molecule in the Pathogenesis of Drug-Induced and I-Cell Disease-Mediated Gingival Overgrowth: A Study with Cathepsin-L-Deficient Mice Am. J. Pathol., December 1, 2002; 161(6): 2047 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hashmi, C. Britton, J. Liu, D. B. Guiliano, Y. Oksov, and S. Lustigman Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans J. Biol. Chem., January 25, 2002; 277(5): 3477 - 3486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Caputo, G. Manco, L. Mandrich, and J. Guardiola A Novel Aspartyl Proteinase from Apocrine Epithelia and Breast Tumors J. Biol. Chem., March 10, 2000; 275(11): 7935 - 7941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Santamaria, G. Velasco, A. M. Pendas, A. Paz, and C. Lopez-Otin Molecular Cloning and Structural and Functional Characterization of Human Cathepsin F, a New Cysteine Proteinase of the Papain Family with a Long Propeptide Domain J. Biol. Chem., May 14, 1999; 274(20): 13800 - 13809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Santamaria, G. Velasco, A. M. Pendas, A. Fueyo, and C. Lopez-Otin Cathepsin Z, a Novel Human Cysteine Proteinase with a Short Propeptide Domain and a Unique Chromosomal Location J. Biol. Chem., July 3, 1998; 273(27): 16816 - 16823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Diamandis, S. Nadkarni, B. Bhaumik, A. Abdelrahman, D. N. Melegos, G. Borchert, M. H. Black, M. Alonso, A. Salas, J. R. de los Toyos, et al. Immunofluorometric assay of pepsinogen C and preliminary clinical applications Clin. Chem., August 1, 1997; 43(8): 1365 - 1371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Day and D. A. Neufeld Use of Enzyme Overlay Membranes to Survey Proteinase Activity in Frozen Sections: Cathepsin-like and Plasmin-like Activity in Regenerating Newt Limbs J. Histochem. Cytochem., June 1, 1997; 45(6): 779 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Silberman, M. Janulis, and R. M. Schultz Characterization of Downstream Ras Signals That Induce Alternative Protease-dependent Invasive Phenotypes J. Biol. Chem., February 28, 1997; 272(9): 5927 - 5935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sers, U. Emmenegger, K. Husmann, K. Bucher, A.-C. Andres, and R. Schafer Growth-inhibitory Activity and Downregulation of the Class II Tumor-suppressor Gene H-rev107 in Tumor Cell Lines and Experimental Tumors J. Cell Biol., February 24, 1997; 136(4): 935 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Balbin and C. Lopez-Otin Hormonal Regulation of the Human Pepsinogen C Gene in Breast Cancer Cells. IDENTIFICATION OF A CIS-ACTING ELEMENT MEDIATING ITS INDUCTION BY ANDROGENS, GLUCOCORTICOIDS, AND PROGESTERONE J. Biol. Chem., June 21, 1996; 271(25): 15175 - 15181. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
