This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chin, T. Articles by Donahoe, P. K. Search for Related Content PubMed PubMed Citation Articles by Chin, T. Articles by Donahoe, P. K.

Human Müllerian Inhibiting Substance Inhibits Tumor Growth in Vitro and in Vivo¹

Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, 11217, Republic of China [T. C.]; Department of Surgery, National Naval Medical Center, Bethesda, Maryland 14853 [R. L. P.]; and Pediatric Surgical Research Laboratory, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114 [T. L., R. L. P., P. K. D.]

Müllerian inhibiting substance (MIS) causes regression of the müllerian duct in the male fetus. Bovine MIS has been reported to inhibit the growth of some gynecological tumors. Recombinant human MIS (rhMIS) produced in transfected Chinese hamster ovary cells has been highly purified by immunoaffinity chromatography. The introduction of a salt wash prior to elution of MIS from the affinity column removes a growth-stimulating factor(s) derived from Chinese hamster ovary cells. This immunopurified rhMIS caused significant inhibition (34–59% survival) of A431 (a vulvar epidermoid carcinoma), HT-3 (a cervical carcinoma), HEC-1-A (an endometrial adenocarcinoma), NIH:OVCAR-3 (an ovarian adenocarcinoma), and OM431 (an ocular melanoma) human cell lines in colony inhibition assays. Two cell lines, Hep 3B (a hepatocellular carcinoma) and RT4 (a bladder transitional cell papilloma), were unresponsive to immunopurified rhMIS. Using an in vivo subrenal capsule assay in irradiated CD-1 mice, the growth of A431 and OM431 cells was inhibited by immunopurified rhMIS. We conclude that rhMIS inhibits the growth of certain tumor cell lines in vitro and in vivo.

¹ This work was supported by National Cancer Institute Grant CA 17393 and American Cancer Society Grant PDT63048 to P. K. D.

² To whom requests for reprints should be addressed, at Pediatric Surgical Research Laboratory, Warren 11, Massachusetts General Hospital, Boston, MA 02114.

Received 11/ 5/90. Accepted 2/ 6/91.

This article has been cited by other articles:

				A. L. Marca and A. Volpe The Anti-Mullerian hormone and ovarian cancer Hum. Reprod. Update, May 1, 2007; 13(3): 265 - 273. [Abstract] [Full Text] [PDF]

				R. Pieretti-Vanmarcke, P. K. Donahoe, L. A. Pearsall, D. M. Dinulescu, D. C. Connolly, E. F. Halpern, M. V. Seiden, and D. T. MacLaughlin Mullerian Inhibiting Substance enhances subclinical doses of chemotherapeutic agents to inhibit human and mouse ovarian cancer PNAS, November 14, 2006; 103(46): 17426 - 17431. [Abstract] [Full Text] [PDF]

				Q.-a. Yuan, H. H. Simmons, M. K. Robinson, M. Russeva, W. A. Marasco, and G. P. Adams Development of engineered antibodies specific for the Mullerian inhibiting substance type II receptor: a promising candidate for targeted therapy of ovarian cancer. Mol. Cancer Ther., August 1, 2006; 5(8): 2096 - 2105. [Abstract] [Full Text] [PDF]

				A. E. Stephen, L. A. Pearsall, B. P. Christian, P. K. Donahoe, J. P. Vacanti, and D. T. MacLaughlin Highly Purified Mullerian Inhibiting Substance Inhibits Human Ovarian Cancer in Vivo Clin. Cancer Res., August 1, 2002; 8(8): 2640 - 2646. [Abstract] [Full Text] [PDF]

				J. Teixeira, S. Maheswaran, and P. K. Donahoe Mullerian Inhibiting Substance: An Instructive Developmental Hormone with Diagnostic and Possible Therapeutic Applications Endocr. Rev., October 1, 2001; 22(5): 657 - 674. [Abstract] [Full Text] [PDF]

				A. E. Stephen, P. T. Masiakos, D. L. Segev, J. P. Vacanti, P. K. Donahoe, and D. T. MacLaughlin Tissue-engineered cells producing complex recombinant proteins inhibit ovarian cancer in vivo PNAS, February 22, 2001; (2001) 51625998. [Abstract] [Full Text]

				N. Morikawa, T. R. Clarke, C. D. Novina, K. Watanabe, C. Haqq, M. Weiss, A. L. Roy, and P. K. Donahoe Human Mullerian-Inhibiting Substance Promoter Contains a Functional TFII-I-Binding Initiator Biol Reprod, October 1, 2000; 63(4): 1075 - 1083. [Abstract] [Full Text]

				P. T. Masiakos, D. T. MacLaughlin, S. Maheswaran, J. Teixeira, A. F. Fuller Jr., P. C. Shah, D. J. Kehas, M. K. Kenneally, D. M. Dombkowski, T. U. Ha, et al. Human Ovarian Cancer, Cell Lines, and Primary Ascites Cells Express the Human Mullerian Inhibiting Substance (MIS) Type II Receptor, Bind, and Are Responsive to MIS Clin. Cancer Res., November 1, 1999; 5(11): 3488 - 3499. [Abstract] [Full Text] [PDF]

				C. M. HAQQ and P. K. DONAHOE Regulation of Sexual Dimorphism in Mammals Physiol Rev, January 1, 1998; 78(1): 1 - 33. [Abstract] [Full Text] [PDF]

				D. L. Segev, T. U. Ha, T. T. Tran, M. Kenneally, P. Harkin, M. Jung, D. T. MacLaughlin, P. K. Donahoe, and S. Maheswaran Mullerian Inhibiting Substance Inhibits Breast Cancer Cell Growth through an NFkappa B-mediated Pathway J. Biol. Chem., September 8, 2000; 275(37): 28371 - 28379. [Abstract] [Full Text] [PDF]

				T. U. Ha, D. L. Segev, D. Barbie, P. T. Masiakos, T. T. Tran, D. Dombkowski, M. Glander, T. R. Clarke, H. K. Lorenzo, P. K. Donahoe, et al. Mullerian Inhibiting Substance Inhibits Ovarian Cell Growth through an Rb-independent Mechanism J. Biol. Chem., November 17, 2000; 275(47): 37101 - 37109. [Abstract] [Full Text] [PDF]

				A. E. Stephen, P. T. Masiakos, D. L. Segev, J. P. Vacanti, P. K. Donahoe, and D. T. MacLaughlin Tissue-engineered cells producing complex recombinant proteins inhibit ovarian cancer in vivo PNAS, March 13, 2001; 98(6): 3214 - 3219. [Abstract] [Full Text] [PDF]