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Comparative Study of Monoclonal Antibodies TURP-27 and HNK-1: Their Relationship to Neural Cell Adhesion Molecules and Prostate Tumor-associated Antigens¹

Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk, Virginia 23501

The murine monoclonal antibodies TURP-27 and HNK-1 have been shown to detect antigens which are heavily expressed by benign prostatic hyperplasia and carcinoma of the prostate. Western blot analysis of prostate extracts showed that monoclonal antibodies TURP-27 and HNK-1 bound glycoproteins with molecular weights of 180,000, 140,000, 120,000, 100,000, 90,000, and 69,000. Studies have shown that the HNK-1 carbohydrate epitope may be involved in cell adhesion and that it is a component of several characterized adhesion proteins. TURP-27 was found to bind at least three of these adhesion proteins: neural cell adhesion molecules; myelin-associated glycoprotein; and a second myelin glycoprotein, P₀. Western blot analysis of prostate extracts showed that an antineural cell adhesion molecule serum bound the M_r 180,000 and 140,000 proteins. Based on reciprocal blocking and chemical tests, it was determined that the TURP-27 and HNK-1 epitopes are not identical. These data imply that the TURP-27 epitope may be a variant of the HNK-1 epitope or that the two epitopes are closely linked and that the TURP-27 and HNK-1 epitopes on prostate cells are positioned on neural cell adhesion molecule-like proteins.

¹ This investigation was supported by NIH Grant CA-26659.

² Present address: Institut für Med. Mikrobiologie und Hygiene, Klinikum rechts der Isar, der Technischen Universität, Trogerstrasse 9, 8000 Munich 80, Federal Republic of Germany.

³ To whom requests for reprints should be addressed, at Department of Microbiology, Eastern Virginia Medical School, P.O. Box 1980, Norfolk, VA 23501.

Received 11/ 8/90. Accepted 2/22/91.

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