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Reactivity of Anti-CD15 Monoclonal Antibody PM-81 with Breast Cancer and Elimination of Breast Cancer Cells from Human Bone Marrow by PM-81 and Immunomagnetic Beads¹

Departments of Medicine [E. D. B., J. J. V.], Microbiology [W. S.], and Pathology [V. A. M.], Dartmouth Medical School, Hanover, New Hampshire 03756

The CD15 carbohydrate antigen, lacto-N-fucopentaose III is expressed on a variety of human cancer cells including acute myeloid leukemia, small cell carcinoma of the lung, and colorectal carcinomas. We have found that cells from breast cancer cell lines and patient-derived tissue are strongly CD15 positive, as seen by binding to the PM-81 monoclonal antibody. In this report we show that monoclonal antibody PM-81 and immunomagnetic beads can remove breast cancer cells from bone marrow and thus be used as "purging" agents for autologous bone marrow transplantation. PM-81 and immunomagnetic beads removed up to 3 log of SK-BR-3 and MCF7 breast carcinoma cell line cells while minimally affecting normal hematopoietic progenitor cells. This technique may be useful for purging marrow for autologous bone marrow transplantation in breast cancer.

¹ Supported in part by Grants CA 37868 from the National Cancer Institute, DHHS, and ACS IN-157 from the American Cancer Society. J. J. V. was a Tiffany Blake Fellow at the Dartmouth-Hitchcock Medical Center. E. D. B. is a Scholar of the Leukemia Society of America.

² To whom requests for reprints should be addressed, at Department of Medicine, Dartmouth Medical School, Hanover, NH 03756.

Received 10/26/90. Accepted 2/22/91.