This Article Full Text (PDF) Erratum (v52,p6137) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shoemaker, R. H. Articles by Boyd, M. R. Search for Related Content PubMed PubMed Citation Articles by Shoemaker, R. H. Articles by Boyd, M. R.

Evaluation of Metastatic Human Tumor Burden and Response to Therapy in a Nude Mouse Xenograft Model Using a Molecular Probe for Repetitive Human DNA Sequences

Laboratory of Drug Discovery Research and Development, Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer Institute [R. H. S., A. M. S., L. W., M. R. B.], and Program Resources Incorporated, National Cancer Institute-Frederick Cancer Research and Development Center [M. S. B.], Frederick, Maryland 21702

A sensitive DNA dot-blot assay for repetitive human DNA sequences was developed and applied to the quantitative determination of spontaneous metastases of a human melanoma in various tissues of nude mice. The assay was useful for defining the time course and pattern of tissue distribution of metastatic cells as well as for assessing response to therapy. The methodology is relatively simple, can be performed using nonradioactive DNA probes, and should be broadly applicable to studies of metastasis of human tumors in nude mice.

¹ To whom requests for reprints should be addressed, at Laboratory of Drug Discovery Research and Development, Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer Institute-Frederick Cancer Research and Development Center, Building 1052, Room 121, Frederick, MD 21702-1201.

Received 10/30/91. Accepted 3/11/92.