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Thermotolerance in the Absence of Induced Heat Shock Proteins in a Murine Lymphoma¹

Department of Radiation Oncology, Stanford University, Stanford, California 94305 [B. F., P. K., G. M. H.], and Peter MacCallum Cancer Institute, 481 Lt. Lonsdale St., Melbourne, Victoria 300, Australia [R. L. A.]

Three murine lymphoma cell lines, CH1, a B-cell lymphoma, and VL3 and RDM4, both T-cell lymphomas, were tested for their ability to induce heat shock protein synthesis and thermotolerance after heat shock. All three lines could develop thermotolerance, but the persistence of tolerance was less than can be measured in nonlymphoid cell lines. Analysis of protein synthesis patterns by one-dimensional gel electrophoresis suggested that only the VL3 cells were capable of the induction of heat shock proteins. After two-dimensional gel analysis, however, the induction of one heat shock protein was evident in RDM4 cells. No induced heat shock proteins could be detected in the CH1 cells. These data provide strong evidence that, while the induction of heat shock proteins may be sufficient for development of thermotolerance, they are not necessary and that another mechanism is available to cells.

¹ Supported by Grant CA44665 from the National Cancer Institute and by the Anti-Cancer Council of Victoria, Australia.

² Present address: London Regional Cancer Centre, 790 Commissioners Rd. E., London, Ontario N6A 4L6, Canada.

³ To whom requests for reprints should be addressed.

Received 3/26/90. Accepted 3/10/92.

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