This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wyllie, F. S. Articles by Wynford-Thomas, D. Search for Related Content PubMed PubMed Citation Articles by Wyllie, F. S. Articles by Wynford-Thomas, D.

A Phenotypically and Karyotypically Stable Human Thyroid Epithelial Line Conditionally Immortalized by SV40 Large T Antigen¹

CRC Thyroid Tumour Biology Research Group, Department of Pathology [F. S. W., J. A. B., T. D., D. W-T.], and Department of Hematology [D. W.], University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, and Department of Medicine, Clinical Sciences Centre, Northern General Hospital, Sheffield S5 7AU [R. D.], United Kingdom

Primary cultures of normal human neonatal thyroid follicular cells were transfected with a plasmid expressing a temperature-sensitive (tsA58) mutant of SV40 large T antigen. An epithelial cell line, designated B-thy-ts.1, was obtained which showed tight temperature-dependent growth. In sharp contrast to previous such lines, which were derived from adult thyroid, B-thy-ts.1 has retained a well-differentiated phenotype as reflected in its morphology and cytokeratin expression pattern. In addition to phenotypic stability the line also displays an unusually stable karyotype, lacking the usual clastogenic effects of SV40, which we speculate to result from a greater DNA repair capacity of its cell of origin. B-thy-ts.1 should be a particularly useful tool with which to study the effects of activated oncogenes on epithelial growth and differentiation.

¹ We gratefully acknowledge the support of the United Kingdom Cancer Research Campaign, the Medical Research Council, and the British Medical Association.

² To whom requests for reprints should be addressed.

Received 10/28/91. Accepted 3/10/92.

This article has been cited by other articles:

				J. Ham, J. Webster, J. A. Bond, B. Jasani, M. D. Lewis, P. J. Hepburn, J. S. Davies, B. M. Lewis, D. W. Thomas, and M. F. Scanlon Immortalized Human Pituitary Cells Express Glycoprotein {alpha}-Subunit and Thyrotropin {beta} (TSH{beta}) J. Clin. Endocrinol. Metab., May 1, 1998; 83(5): 1598 - 1603. [Abstract] [Full Text]