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Expression of the Epidermal Growth Factor Receptor in Human Small Cell Lung Cancer Cell Lines¹

Institute of Pathological Anatomy, University of Copenhagen [L. D., K. R., M. S. T., H. S. P], and Department of Oncology, Rigshospitalet [L. D., H. S. P.], DK-2100 Copenhagen, Denmark

Epidermal growth factor (EGF) receptor expression was evaluated in a panel of 21 small cell lung cancer cell lines with radioreceptor assay, affinity labeling, and Northern blotting. We found high-affinity receptors to be expressed in 10 cell lines. Scatchard analysis of the binding data demonstrated that the cells bound between 3 and 52 fmol/mg protein with a K_D ranging from 0.5 x 10^-10 to 2.7 x 10^-10 M. EGF binding to the receptor was confirmed by affinity-labeling EGF to the EGF receptor. The cross-linked complex had a M_r of 170,000–180,000. Northern blotting showed the expression of EGF receptor mRNA in all 10 cell lines that were found to be EGF receptor-positive and in one cell line that was found to be EGF receptor-negative in the radioreceptor assay and affinity labeling. Our results provide, for the first time, evidence that a large proportion of a broad panel of small cell lung cancer cell lines express the EGF receptor.

¹ This work was supported by the Danish Cancer Society, the Danish Research Academy, the Mogens and Jenny Vissing Foundation, the Ellen Hindsgaul Foundation, and the Jacob Madsen Foundation.

² To whom requests for reprints should be addressed, at Department of Oncology 5074, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.

Received 10/ 7/91. Accepted 3/24/92.

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