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Expression of c-sis/Platelet-derived Growth Factor B, Insulin-like Growth Factor I, and Transforming Growth Factor Messenger RNAs and Their Respective Receptor Messenger RNAs in Primary Human Gastric Carcinomas: In Vivo Studies with in Situ Hybridization and Immunocytochemistry.¹

The Center for Blood Research, and the Departments of Cancer Biology and Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115

In situ hybridization and immunocytochemistry have been applied to investigate the expression of c-sis/platelet-derived growth factor (PDGF)-B, insulin-like growth factor (IGF)-I, and transforming growth factor mRNAs and their respective receptor mRNAs in three primary human gastric carcinomas and in their adjacent nonmalignant mucosas. Expression of c-sis/PDGF-B mRNA and PDGF-receptor ß mRNA was seen in the tumor cells of the three gastric cancer specimens but not in their adjacent nonmalignant mucosa. The mRNA expression was accompanied by the expression of their respective protein products. IGF-I, IGF-I receptor, and epidermal growth factor receptor mRNAs were seen in both the tumor cells of the gastric cancer specimens and in nonmalignant mucosa. Transforming growth factor mRNA was expressed in gastric tumor cells but not in nomalignant mucosa. The coexpression of a potent "competence" growth factor, PDGF, and "progression" growth factors, IGF-I and transforming growth factor , in the tumor cells of gastric carcinomas may contribute to their growth and maintenance.

¹ This work was supported by Grants CA30101 and HL29583 from NIH (H. N. A.) and the Council for Tobacco Research, USA, Inc. (H. N. A.).

² Supported by a grant from the Korea Science and Engineering Foundation (KOSEF). Dr. Chung is a visiting scholar in the Department of Nutrition, Harvard School of Public Health. Present address: Hallym University, Department of Food and Nutrition, 1 Okchon-Dong, Chunchon 200, Korea.

³ To whom requests for reprints should be addressed, at The Center for Blood Research, 800 Huntington Avenue, Boston, MA 02115.

Received 11/14/91. Accepted 4/ 3/92.

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