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p53 Mutations in Basal Cell Carcinomas¹

Departments of Microbiology [P. R., F. S., S. K. T.] and Dermatology [R. W., S. K. T.], University of Texas Medical Branch, Galveston, Texas 77555-1019

Genomic DNA from 14 basal cell carcinoma biopsies was screened for the presence of mutations in the p53 gene, using the polymerase chain reaction followed by direct DNA sequencing. Heterozygous mutations were detected in 7 of 14 (50%) samples investigated. All mutations were G:C-A:T transitions, and five (71%) of these mutations were transitions at hot spots with CpG sites, three at codon 248 and two at codon 273. The striking similarity of the type of mutations detected in this study and with the UV mutagenesis studies reported in literature suggest the hypothesis that UV may act on the p53 gene in a carcinogenic-specific fashion.

¹ This work was supported in part by The National Institute of Allergy and Infectious Disease.

² Present address: Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.

³ To whom requests for reprints should be addressed, at the Department of Microbiology, University of Texas Medical Branch, Route J19, Galveston, TX 77555-1019.

Received 3/30/92. Accepted 5/13/92.

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