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An Altered 11-Kilobase Transcript in Leukemic Cell Lines with the t(4;11)(q21;q23) Chromosome Translocation¹

Jefferson Cancer Institute, Jefferson Cancer Center and Department of Microbiology and Immunology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107 [G. C., T. N., Y. G., O. C., R. P., C. M. C., E. C.]; St. Jude Children's Research Hospital, and the University of Tennessee, Memphis College of Medicine, Memphis, Tennessee 38101 [W. M. C.]; University of Alabama at Birmingham, Birmingham, Alabama 35294 [A. J. C.]; the Pediatric Oncology Group, St. Louis, Missouri 63108 [W. M. C. and A. J. C.]; Roswell Park Cancer Institute, Buffalo, New York 14623 [M. B., C. D. B.]; and University of Pennsylvania, Philadelphia, Pennsylvania 19104 [P. C. N.]

The 11q23 chromosome band is frequently associated with chromosomal aberrations in human leukemias. We have previously cloned a DNA fragment derived from chromosome 11 which could be used as a probe to detect rearrangements in DNAs from the leukemic cells of patients with the t(4;11), t(9;11), and t(11;19) translocations. In this study we now show that the same probe detects DNA rearrangements in malignant cells from patients with the t(1;11), t(6;11), t(10;11), and del (11q23) chromosomal abnormalities. A second probe obtained from a region located centrometric to the breakpoint cluster detects major and minor transcripts of 12.5 and 11.5 kilobases, respectively, in all cell lines examined. The same probe identifies an altered 11-kilobase RNA in all three independent cell lines with the t(4;11)(q21;q23) chromosome translocation.

¹ Supported by an Outstanding Investigation Grant (CA39860) to C. M. C. from the National Cancer Institute and National Cancer Institute Grants CA30969, CA31566, and CA21765.

² To whom requests for reprints should be addressed.

Received 4/28/92. Accepted 5/26/92.

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