This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilson, D. F. Articles by Cerniglia, G. J. Search for Related Content PubMed PubMed Citation Articles by Wilson, D. F. Articles by Cerniglia, G. J.

Localization of Tumors and Evaluation of Their State of Oxygenation by Phosphorescence Imaging¹

Departments of Biochemistry and Biophysics [D. F. W.] and of Radiation Oncology [G. J. C.], Medical School, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Oxygen-dependent quenching of phosphorescence has been used to image the distribution of oxygen pressure in small tumors and surrounding tissue. Suspensions of cultured 9L cells or small pieces of solid tumors from 9L cells were injected into the surface of the muscle of the hindquarter of rats, and the tumors were grown until they were 0.2–1.0 cm in diameter. The phosphorescent probe for oxygen was injected into the systemic blood, and phosphorescence was imaged with a video camera. Images of the phosphorescence were collected using a series of different delay times after illumination with a light flash (<5-µs width at half-height), and the phosphorescence decay constants (lifetimes) and oxygen pressure were calculated for each pixel of the image arrays. The areas of tissue within the tumors were observed to have increased phosphorescence lifetimes and lower oxygen pressures than the surrounding tissue. Phosphorescence imaging is, therefore, a noninvasive optical method which permits quantitation of the distribution of oxygen in small tumors and also, at least in the 9L tumors, differentiation of tumor from normal tissue.

¹ This work was supported by NIH Grants NS-10939 (to D. F. W.) and CA-44982 (to Dr. John Biaglow).

² To whom requests for reprints should be addressed, at Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104.

Received 9/20/91. Accepted 5/ 1/92.

This article has been cited by other articles:

				Y. Kabe, M. Ohmori, K. Shinouchi, Y. Tsuboi, S. Hirao, M. Azuma, H. Watanabe, I. Okura, and H. Handa Porphyrin Accumulation in Mitochondria Is Mediated by 2-Oxoglutarate Carrier J. Biol. Chem., October 20, 2006; 281(42): 31729 - 31735. [Abstract] [Full Text] [PDF]

				M. Shibata, S. Ichioka, and A. Kamiya Estimating oxygen consumption rates of arteriolar walls under physiological conditions in rat skeletal muscle Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H295 - H300. [Abstract] [Full Text] [PDF]

				K. Tsukada, E. Sekizuka, C. Oshio, K. Tsujioka, and H. Minamitani Red blood cell velocity and oxygen tension measurement in cerebral microvessels by double-wavelength photoexcitation J Appl Physiol, April 1, 2004; 96(4): 1561 - 1568. [Abstract] [Full Text] [PDF]

				G. Schears, S. E. Schultz, J. Creed, W. J. Greeley, D. F. Wilson, and A. Pastuszko Effect of perfusion flow rate on tissue oxygenation in newborn piglets during cardiopulmonary bypass Ann. Thorac. Surg., February 1, 2003; 75(2): 560 - 565. [Abstract] [Full Text] [PDF]

				M. Shibata, S. Ichioka, J. Ando, and A. Kamiya Microvascular and interstitial PO2 measurements in rat skeletal muscle by phosphorescence quenching J Appl Physiol, July 1, 2001; 91(1): 321 - 327. [Abstract] [Full Text] [PDF]

				M. Hockel and P. Vaupel Tumor Hypoxia: Definitions and Current Clinical, Biologic, and Molecular Aspects J Natl Cancer Inst, February 21, 2001; 93(4): 266 - 276. [Abstract] [Full Text] [PDF]

				R. Motterlini, H. Kerger, C. J. Green, R. M. Winslow, and M. Intaglietta Depression of endothelial and smooth muscle cell oxygen consumption by endotoxin Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H776 - H782. [Abstract] [Full Text] [PDF]

				G. J. Cerniglia, D. F. Wilson, M. Pawlowski, S. Vinogradov, and J. Biaglow Intravascular oxygen distribution in subcutaneous 9L tumors and radiation sensitivity J Appl Physiol, June 1, 1997; 82(6): 1939 - 1945. [Abstract] [Full Text] [PDF]