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Multiparameter Assessment of the Cell Cycle Effects of Bioactive and Cytotoxic Agents¹

University of Cincinnati Medical Center, Cincinnati, Ohio 45267-0508

This paper describes the use of the bromodeoxyuridine/propidium iodide method to assess the effects of bioactive and cytotoxic agents on the kinetic characteristics of acute myelogenous leukemia cells. By careful selection of gates, the following parameters can be measured simultaneously using only 50,000 cells: the proportion of cells in S-phase, the distribution of cells within the S-phase compartment, the relative rate of DNA synthesis, the relative distribution of S-phase times, the proportion of S_o cells, and the proportion of cells in G₁ and G₂/M. This method was used to demonstrate that while retinoic acid, -interferon, and cytosine arabinoside may all "inhibit" DNA synthesis, the actual effects of these agents differ. Retinoic acid appears to arrest cells in G₁ without affecting the rate of DNA synthesis, while -interferon and cytosine arabinoside "inhibit" DNA synthesis by reducing the rate of synthesis per se.

¹ This work was supported by National Cancer Institute Grant CA 41285.

² To whom requests for reprints should be addressed, at University of Cincinnati Medical Center, 231 Bethesda Avenue, M.L. 508, Cincinnati, OH 45267-0508.

Received 1/ 2/92. Accepted 5/12/92.

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