This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Raschellà, G. Articles by Calabretta, B. Search for Related Content PubMed PubMed Citation Articles by Raschellà, G. Articles by Calabretta, B.

Inhibition of Proliferation by c-myb Antisense RNA and Oligodeoxynucleotides in Transformed Neuroectodermal Cell Lines¹

ENEA (Ente Nuove technologie Energia e Ambiente) CRE Casaccia, Division of Molecular Biology, Via Anguillarese, 303, 00060 Rome, Italy [G. R., A. N., S. P., A. R.], and the Department of Microbiology and Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 [T. S., M. N-S., B. C.]

Transfection of a neuroblastoma cell line with expression vectors containing two different segments of human c-myb complementary DNA in antisense orientation yielded far fewer transfectant clones than did the transfection with the identical segments in sense orientation. In cell clones expressing c-myb antisense RNA, levels of the c-myb protein were down-regulated and the proliferation rate was slower than that of cells transfected with sense constructs or the untransfected parental cell lien. Treatment of neuroblastoma and neuroepithelioma cell lines with a c-myb antisense oligodeoxynucleotide strongly inhibited cell growth. These data indicate a definite involvement of c-myb in the proliferation of neuroectodermal tumor cells extending the role of this protooncogene beyond the hematopoietic system. The availability of cell clones that transcribe c-myb antisense RNA provides a useful tool to study the involvement of other genes in the proliferation and differentiation of neuroblastoma cells.

¹ This work was supported in part by NIH Grant CA46782, by American Cancer Society Grant CH-455A, and by a grant from the Associazione Italiana Ricerca sul Cancro (AIRC).

² To whom requests for reprints should be addressed, at ENEA (Ente technologie Energia e Ambiente) CRE Casaccia, Division of Molecular Biology, Via Anguillarese, 303, 00060 Rome, Italy (GR), or Department of Microbiology, Thomas Jefferson University, 10th and Locust Streets, Philadelphia, PA 19107 (BC).

³ Scholar of the Leukemia Society of America.

Received 12/13/91. Accepted 5/27/92.

This article has been cited by other articles:

				C. Brignole, F. Pastorino, D. Marimpietri, G. Pagnan, A. Pistorio, T. M. Allen, V. Pistoia, and M. Ponzoni Immune Cell-Mediated Antitumor Activities of GD2-Targeted Liposomal c-myb Antisense Oligonucleotides Containing CpG Motifs J Natl Cancer Inst, August 4, 2004; 96(15): 1171 - 1180. [Abstract] [Full Text] [PDF]

				S. Laurora, S. Pizzimenti, F. Briatore, A. Fraioli, M. Maggio, P. Reffo, C. Ferretti, M. U. Dianzani, and G. Barrera Peroxisome Proliferator-Activated Receptor Ligands Affect Growth-Related Gene Expression in Human Leukemic Cells J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 932 - 942. [Abstract] [Full Text] [PDF]

				B. Tanno, A. Negroni, R. Vitali, M. C. Pirozzoli, V. Cesi, C. Mancini, B. Calabretta, and G. Raschella Expression of Insulin-like Growth Factor-binding Protein 5 in Neuroblastoma Cells Is Regulated at the Transcriptional Level by c-Myb and B-Myb via Direct and Indirect Mechanisms J. Biol. Chem., June 21, 2002; 277(26): 23172 - 23180. [Abstract] [Full Text] [PDF]

				G. Pagnan, D. D. Stuart, F. Pastorino, L. Raffaghello, P. G. Montaldo, T. M. Allen, B. Calabretta, and M. Ponzoni Delivery of c-myb Antisense Oligodeoxynucleotides to Human Neuroblastoma Cells Via Disialoganglioside GD2-Targeted Immunoliposomes: Antitumor Effects J Natl Cancer Inst, February 2, 2000; 92(3): 253 - 261. [Abstract] [Full Text] [PDF]

				G. Raschella, V. Cesi, R. Amendola, A. Negroni, B. Tanno, P. Altavista, G. Paolo Tonini, B. De Bernardi, and B. Calabretta Expression of B-myb in Neuroblastoma Tumors Is a Poor Prognostic Factor Independent from MYCN Amplification Cancer Res., July 1, 1999; 59(14): 3365 - 3368. [Abstract] [Full Text] [PDF]

				G. Raschell, A. Negroni, A. Sala, S. Pucci, A. Romeo, and B. Calabretta Requirement of B- myb Function for Survival and Differentiative Potential of Human Neuroblastoma Cells J. Biol. Chem., April 14, 1995; 270(15): 8540 - 8545. [Abstract] [Full Text] [PDF]