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Elevated Membrane Association of Phospholipase C-1 in MDA-468 Mammary Tumor Cells¹

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville 37232-0146 [A. M. S., G. C., G. T.], and Department of Medicine, Division of Dermatology, Vanderbilt University School of Medicine, Nashville 37232-2358 [G. C.], Tennessee

We have examined the distribution of phospholipase C-1 (PLC-1) between membrane and cytosolic fractions in several cell lines. In MDA-468 cells, which are derived from a human breast tumor, greater than one-half of the total PLC-1 is associated with the membrane fraction of the cell. Unlike the situation in A-431 cells [G. Todderud, M. I. Wahl, S. G. Ree, and G. Carpenter, Science, 248: 296–298, 1990], epidermal growth factor (EGF) stimulation of MDA-468 cells does not result in significantly increased PLC-1 association with membranes. Immunoblot analysis reveals low levels of phosphotyrosine in PLC-1 and EGF receptors in unstimulated MDA-468 cells and greatly increased phosphotyrosine levels in these proteins as a result of EGF stimulation of the cells. We conclude that autocrine activation of EGF receptors is not responsible for the elevated association of PLC-1 with membranes in these cells.

¹ This work was supported by NIH Grants CA43720 and AM07491.

² Present address: Bristol-Myers Squibb, Pharmaceutical Research Institute, 100 Forest Ave., Buffalo, NY 14213-1091.

³ To whom requests for reprints should be addressed.

Received 3/27/92. Accepted 6/ 8/92.

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