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Laboratory of Cellular and Molecular Biology, National Cancer Institute [T. P. F., S. A. A.] and Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke [A. S., E. H. O., I. U. A.], NIH, Bethesda, Maryland 20892 and Department of Neurosurgery, University of Tennessee, Memphis, Tennessee 38119 [W. C. C., J. T. R.]
Analysis of genomic organization and expression of platelet-derived growth factor receptors (PDGFR) and epidermal growth factor receptor (EGFR) in human malignant gliomas showed amplification and over-expression of both receptors in distinct subsets of tumors. Amplification of the 
PDGFR was detected in 4 of 50 glioblastomas (8%). EGFR was amplified in 9 of the 50 tumors (18%). Western blot analysis showed elevated expression of PDGFR and EGFR proteins in 4 (24%) and 3 (18%), respectively, of 17 tumor specimens analyzed. Increased production of PDGFR as well as EGFR proteins was observed in the presence or absence of gene amplification. Three of the 4 tumors with elevated levels of PDGFR also overexpressed the ßPDGFR, which was present as a single copy gene in all 50 tumors analyzed. Our findings suggest that the amplification and/or overexpression either of EGFR or of the PDGFR along with the coordinate overexpression of the ßPDGFR can contribute to the malignant phenotype of distinct subsets of human glioblastoma.
1 Present address: Department of Ophthalmology and Visual Sciences, Washington University, School of Medicine, St. Louis, MO 63110.
2 To whom requests for reprints should be addressed.
Received 6/23/92. Accepted 7/ 2/92.
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