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In Vivo and in Vitro Growth-inhibitory Effect of Bovine Seminal Ribonuclease on a System of Rat Thyroid Epithelial Transformed Cells and Tumors¹

Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Reggio Calabria, Facoltà di Medicina e Chirurgia di Catanzaro, via Tommaso Campanella 88100 Catanzaro [P. L.]; Istituto Nazionale dei Tumori "Fondazione G. Pascale," via M. Semmola, 80131 Napoli [G. P.]; Dipartimento di Chimica Organica e Biologica, Università degli Studi di Napoli, Facoltà di Scienze, via Mezzocannone 16, 80134 Napoli [M. R. M., A. R., R. P., G. D.]; and Centro di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università degli Studi di Napoli, II Facoltà di Medicina e Chirurgia, via S. Pansini 5, 80131 Napoli [G. V.]; Italy

We investigated the antitumoral effect of bovine seminal RNase (BS-RNase) in vivo and in vitro on a model system of epithelial tumor- and metastasis-derived cells as well as on epithelial tumors derived from the same system. We found that while BS-RNase significantly inhibited the growth in vitro of the epithelial tumor-derived cells, its inhibitory effect was even more dramatic on the growth of metastasis-derived cells. BS-RNase exerted no appreciable growth inhibition on normal thyroid epithelial cells. When administered in vivo to rats bearing solid carcinomas, having the same thyroid origin, BS-RNase induced a drastic reduction in the tumor weight, with no detectable toxic effects on the treated animals. These data show, for the first time on a system of neoplastically transformed epithelial cells, that BS-RNase has a potent specific antitumoral activity.

¹ This work was supported by the Associazione Italiana per la Ricerca sul Cancro and by the CNR Progetto Finalizzato "Biotecnologie e Biostrumentazione 70/9108525 and 9101161." A. R. is a recipient of a fellowship from the Associazione Italiana per la Ricerca sul Cancro.

² To whom requests for reprints should be addressed.

Received 3/ 4/92. Accepted 6/17/92.

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