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Analysis of T-Cell Receptor /ß Variability in Lymphocytes Infiltrating a Melanoma Metastasis¹

Laboratoire d'Hémato-Immunologie, INSERM U333, Institut Gustave Roussy, 94805 Villejuif Cedex, France

Multiple experimental and clinical studies have suggested that the immune system may, to some extent, control the development of melanomas. The presence of tumor-infiltrating lymphocytes could reflect an in situ immune reaction directed to the malignant cells. The characterization of T-cell receptor (TCR) expressed by tumor-infiltrating lymphocytes is one way to precisely analyze these local T-cell responses. In this study, we have assessed the TCR /ß variability in tumor-infiltrating lymphocytes from a subcutaneous metastasis of a melanoma patient. Using the anchored-polymerase chain reaction 268 TCR and 266 TCR ß chain transcripts have been cloned and sequenced. Their analysis shows that the T-cell infiltrate is extremely diverse, with no preferential TCR gene segment usage.

¹ Supported in part by ADRC Grants 2110 and 6338.

² To whom requests for reprints should be addressed, at Laboratoire d'Hémato-Immunologie, INSERM U333, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France.

Received 1/15/92. Accepted 6/23/92.

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