| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Division of Hematology/Oncology, Departments of Pediatrics [L. A. M., S. G. E.] and Internal Medicine [S. G. E.] University of Michigan, Ann Arbor, Michigan 48109-0680; and Pediatric Bone Marrow Transplant Unit, Ahmanson Department of Pediatrics, Steven Spielberg Pediatric Research Center, Cedars-Sinai Medical Center, Los Angeles, California 90048 [T. J. M.]
The presence of circulating tumor cells in patients with localized or disseminated neuroblastoma may be a significant prognostic factor at diagnosis and may antedate the detection of relapse by other diagnostic studies. We report the development of a highly sensitive detection assay for circulating neuroblasts based on the reverse transcriptase-polymerase chain reaction (RT-PCR), using the neuroendocrine protein gene product 9.5 (PGP 9.5) as the tumor marker. Analysis of RT-PCR products by agarose gel electrophoresis demonstrated that neuroblastoma cell lines were uniformly positive, whereas peripheral blood mononuclear cells were negative. Alkaline Southern blotting with a PGP 9.5-specific probe revealed scant expression of PGP 9.5 in peripheral blood mononuclear cells, well below the limits of detection by electrophoresis alone. The system was able to detect a single neuroblastoma cell in 107 peripheral blood mononuclear cells. Eighteen patient samples were analyzed by PGP 9.5 RT-PCR and the results compared with immunocytology in 16. Ten of the 18 were negative by both studies. Eight of the 18 were positive by PGP 9.5 RT-PCR, 4 of which were also positive by immunocytology. PGP 9.5 RT-PCR was able to detect circulating neuroblasts in two patients with negative immunocytology, the first with progressive bone marrow disease and the second at high risk for relapse but no other evidence of disease. PGP 9.5 RT-PCR allows the detection of circulating neuroblastoma cells with a sensitivity greater than immunocytology. It will be useful in evaluating the clinical significance of circulating tumor cells with respect to prognosis and early detection of relapse, and in the screening of peripheral stem cell harvests prior to autologous infusion.
1 This research was supported by research awards from the United States NIH.
2 Recipient of a Scholar Award from the Leukemia Society of America. To whom requests for reprints should be addressed, at Room 5510B MSRB1, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0680.
Received 2/21/92. Accepted 6/17/92.
This article has been cited by other articles:
|
|
 |
|
  K. Mori, T. Suzuki, H. Uozaki, H. Nakanishi, T. Ueda, Y. Matsuno, Y. Kodera, H. Sakamoto, N. Yamamoto, M. Sasako, et al. Detection of Minimal Gastric Cancer Cells in Peritoneal Washings by Focused Microarray Analysis with Multiple Markers: Clinical Implications Ann. Surg. Oncol., May 1, 2007; 14(5): 1694 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Pathak, M. Bhutani, S. Kumar, A. Mohan, and R. Guleria Circulating Cell-Free DNA in Plasma/Serum of Lung Cancer Patients as a Potential Screening and Prognostic Tool Clin. Chem., October 1, 2006; 52(10): 1833 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Swerts, B. De Moerloose, C. Dhooge, J. Vandesompele, C. Hoyoux, K. Beiske, Y. Benoit, G. Laureys, and J. Philippe Potential Application of ELAVL4 Real-Time Quantitative Reverse Transcription-PCR for Detection of Disseminated Neuroblastoma Cells Clin. Chem., March 1, 2006; 52(3): 438 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Corrias, L. B. Faulkner, A. Pistorio, C. Rosanda, F. Callea, M. S. L. Piccolo, P. Scaruffi, C. Marchi, L. Lacitignola, M. Occhino, et al. Detection of Neuroblastoma Cells in Bone Marrow and Peripheral Blood by Different Techniques: Accuracy and Relationship with Clinical Features of Patients Clin. Cancer Res., December 1, 2004; 10(23): 7978 - 7985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yano, N. Uematsu, T. Yashiro, H. Hara, E. Ueno, M. Miwa, G. Tsujimoto, Y. Aiyoshi, and K. Uchida Gene Expression Profiling Identifies Platelet-Derived Growth Factor as a Diagnostic Molecular Marker for Papillary Thyroid Carcinoma Clin. Cancer Res., March 15, 2004; 10(6): 2035 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Gupta, L. Taguba, R. Arciaga, A. Siperstein, C. Faiman, A. Mehta, and S. S. K. Reddy Detection of Circulating Thyroid Cancer Cells by Reverse Transcription-PCR for Thyroid-stimulating Hormone Receptor and Thyroglobulin: The Importance of Primer Selection Clin. Chem., October 1, 2002; 48(10): 1862 - 1865. [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. B. Hoon, C. T. Kuo, S. Wen, H. Wang, L. Metelitsa, C. P. Reynolds, and R. C. Seeger Ganglioside GM2/GD2 Synthetase mRNA Is a Marker for Detection of Infrequent Neuroblastoma Cells in Bone Marrow Am. J. Pathol., August 1, 2001; 159(2): 493 - 500. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. A. Burchill, I. J. Lewis, K. R. Abrams, R. Riley, J. Imeson, A. D.J. Pearson, R. Pinkerton, and P. Selby Circulating Neuroblastoma Cells Detected by Reverse Transcriptase Polymerase Chain Reaction for Tyrosine Hydroxylase mRNA Are an Independent Poor Prognostic Indicator in Stage 4 Neuroblastoma in Children Over 1 Year J. Clin. Oncol., March 15, 2001; 19(6): 1795 - 1801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Seeger, C. P. Reynolds, R. Gallego, D. O. Stram, R. B. Gerbing, and K. K. Matthay Quantitative Tumor Cell Content of Bone Marrow and Blood as a Predictor of Outcome in Stage IV Neuroblastoma: A Children's Cancer Group Study J. Clin. Oncol., December 15, 2000; 18(24): 4067 - 4076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Faulkner, A. Garaventa, A. Paoli, V. Tintori, A. Tamburini, L. Lacitignola, M. Veltroni, M. S. L. Piccolo, E. Viscardi, C. Milanaccio, et al. In Vivo Cytoreduction Studies and Cell Sorting-Enhanced Tumor-Cell Detection in High-Risk Neuroblastoma Patients: Implications for Leukapheresis Strategies J. Clin. Oncol., November 15, 2000; 18(22): 3829 - 3836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sabbatini, M. Federico, M. Morselli, R. Depenni, K. Cagossi, M. Luppi, G. Torelli, and V. Silingardi Detection of Circulating Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction of Maspin in Patients With Breast Cancer Undergoing Conventional-Dose Chemotherapy J. Clin. Oncol., May 9, 2000; 18(9): 1914 - 1920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, P. Einhorn, T. J. Triche, R. C. Seeger, and C. P. Reynolds Expression of Protein Gene Product 9.5 and Tyrosine Hydroxylase in Childhood Small Round Cell Tumors Clin. Cancer Res., February 1, 2000; 6(2): 551 - 558. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Zhang, S. Kharbanda, S. W. McLeskey, and F. G. Kern Overexpression of Fibroblast Growth Factor 1 in MCF-7 Breast Cancer Cells Facilitates Tumor Cell Dissemination but Does Not Support the Development of Macrometastases in the Lungs or Lymph Nodes Cancer Res., October 1, 1999; 59(19): 5023 - 5029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ylikoski, M. Sjoroos, A. Lundwall, M. Karp, T. Lovgren, H. Lilja, and A. Iitia Quantitative Reverse Transcription-PCR Assay with an Internal Standard for the Detection of Prostate-specific Antigen mRNA Clin. Chem., September 1, 1999; 45(9): 1397 - 1407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Ghossein, S. Bhattacharya, and J. Rosai Molecular Detection of Micrometastases and Circulating Tumor Cells in Solid Tumors Clin. Cancer Res., August 1, 1999; 5(8): 1950 - 1960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mellado, L. Gutierrez, T. Castel, D. Colomer, M. Fontanillas, J. Castro, and J. Estape Prognostic Significance of the Detection of Circulating Malignant Cells by Reverse Transcriptase-Polymerase Chain Reaction in Long-Term Clinically Disease-free Melanoma Patients Clin. Cancer Res., July 1, 1999; 5(7): 1843 - 1848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Tseng, C. C. Pao, J.-D. Lin, Y.-K. Soong, J.-H. Hong, and S. Hsueh Detection of Human Papillomavirus Types 16 and 18 mRNA in Peripheral Blood of Advanced Cervical Cancer Patients and Its Association With Prognosis J. Clin. Oncol., May 1, 1999; 17(5): 1391 - 1391. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
