This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fonagy, A. Articles by Freeman, J. W. Search for Related Content PubMed PubMed Citation Articles by Fonagy, A. Articles by Freeman, J. W.

Antisense-mediated Specific Inhibition of P120 Protein Expression Prevents G₁- to S-Phase Transition¹

Department of Surgery, Division of General Surgery, and Department of Microbiology and Immunology, Lucille Markey Cancer Center, University of Kentucky Medical Center, Lexington, Kentucky 40536

A pentadecadeoxyribonucleotide (5'-AAAGCCCCCCACCAC), complementary to a splice junction site of mRNA for human proliferation-associated nucleolar protein P120, inhibited expression of the P120 gene and the mitogen-induced proliferation of human lymphocytes. The inhibition of P120 gene expression and proliferation was concentration dependent and reached 90% at 200 µM, as measured by [³H]thymidine uptake and by densitometric scanning of Northern (mRNA) and Western (protein) blots of P120. Inhibition was not observed in cells treated with the correspondent nonsense oligomer. P120 antisense oligomer treatment prevented S-phase entry of mitogen-stimulated lymphocytes, as determined by flow cytometric analysis, but did not block G₀-G₁ transition assessed by morphological blast transformation and induction of [³H]uridine incorporation. Results of this study suggest that P120 expression may be required for the upregulation of nucleolar function necessary for cell proliferation.

¹ This work was supported by grant CA49633 from the USPHS-National Cancer Institute.

² To whom requests for reprints should be addressed, at Combs Research Facility, Rm. 313, University of Kentucky Medical Center, 800 Rose Street, Lexington, KY 40536-0093.

Received 3/12/92. Accepted 7/23/92.

This article has been cited by other articles:

				D. G. Pestov, Z. Strezoska, and L. F. Lau Evidence of p53-Dependent Cross-Talk between Ribosome Biogenesis and the Cell Cycle: Effects of Nucleolar Protein Bop1 on G1/S Transition Mol. Cell. Biol., July 1, 2001; 21(13): 4246 - 4255. [Abstract] [Full Text] [PDF]

				C. C. Chou and B. Y. M. Yung Increased Stability of Nucleophosmin/B23 in Anti-Apoptotic Effect of Ras during Serum Deprivation Mol. Pharmacol., January 1, 2001; 59(1): 38 - 45. [Abstract] [Full Text]

				J. Amann, V. J. Kidd, and J. M. Lahti Characterization of Putative Human Homologues of the Yeast Chromosome Transmission Fidelity Gene, CHL1 J. Biol. Chem., February 7, 1997; 272(6): 3823 - 3832. [Abstract] [Full Text] [PDF]