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Frequent Loss of Heterozygosity for Loci on Chromosome 8p in Hepatocellular Carcinoma, Colorectal Cancer, and Lung Cancer¹

Departments of Biochemistry [M. E., Y. F., Y. N.], Surgery [T. N.], and Pathology [E. T.], Cancer Institute, 1-37-1 Kami-Ikebukuro, Toshima-ku, 170; Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, 104 [H. T., S. H.]; Departments of Pathology [Y. M.] and Surgery [K. T.], Tokyo Metropolitan Komagome Hospital; and Department of Biochemistry, Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, 113 [M. M.], Tokyo, Japan

Frequent loss of heterozygosity at chromosomal loci in a specific tumor type may indicate the presence of a tumor suppressor gene. We have examined loss of heterozygosity on chromosome 8p in paired tumor and constitutional DNA from 346 patients representing seven different types of human cancer. Frequent allelic losses were observed in hepatocellular carcinoma (22 of 46 cases, 47.8%), in colorectal cancer (12 of 26, 46.2%), and in non-small cell lung cancer (14 of 35, 40.0%), in contrast to low frequencies detected in breast cancer (5 of 56, 8.9%) and renal cell carcinoma (2 of 27, 7.4%). Ovarian cancer and gastric cancer showed intermediate frequencies of 33.3% and 22.2%. Subsequent analysis of 120 hepatocellular carcinomas and 94 colorectal cancers with five polymorphic markers along the short arm of chromosome 8 defined commonly deleted regions within the same chromosomal interval, 8p23. 1-8p21.3, suggesting that one or more tumor suppressor genes for both cancers may be present in that region.

¹ Supported by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science, and Culture of Japan.

² To whom requests for reprints should be addressed.

Received 5/ 7/92. Accepted 7/20/92.

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