This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pflug, B. R. Articles by Djakiew, D. Search for Related Content PubMed PubMed Citation Articles by Pflug, B. R. Articles by Djakiew, D.

Reduced Expression of the Low Affinity Nerve Growth Factor Receptor in Benign and Malignant Human Prostate Tissue and Loss of Expression in Four Human Metastatic Prostate Tumor Cell Lines¹

Departments of Anatomy and Cell Biology [B. R. P., M. O., D. D.] and Surgery, Division of Urology [J. H. L., D. D.], and The Lombardi Cancer Research Center [J. H. L., D. D.], Georgetown University Medical Center, Washington, D.C. 20007

In the human prostate, a low affinity (p75) nerve growth factor (NGF) receptor (NGF-R) localizes to the epithelia while a NGF-like protein localizes to the stroma. This NGF-like ligand, derived from prostate stromal cell cultures, has been shown to participate in paracrine mediated growth of a human tumor epithelial cell line (TSU-prl) in vitro. In order to investigate the role of the NGF-R in neoplastic growth we have examined the expression of the NGF-R in normal prostate tissues, benign prostatic hyperplasia tissues, adenocarcinoma tissues, and four metastatic tumor cell lines of the human prostate. In primary epithelial cell cultures of normal human prostate the p75 NGF-R was localized by immunocytochemistry to cytoplasmic vesicles. Furthermore, Western blot analysis of the NGF-R in subcellular fractions of normal prostate tissue identified an M_r 75,000 immunoreactive protein in the microsomal fraction under nonreducing conditions of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, microsomal preparations of five prostatic adenocarcinoma and five benign prostatic hyperplasia specimens showed varying immunoreactivity among samples, all of which expressed less of the p75 NGF-R than the normal tissue. Interestingly, microsomal preparations of the human prostatic epithelial cell lines, TSU-prl, DU-145, PC-3, and LNCaP did not show NGF-R expression by immunoblot analysis. Hence, expression of the p75 NGF-R in normal prostate tissue, partial loss of NGF-R expression in benign and malignant prostate tissue, and complete loss of NGF-R expression in the four metastatic tumor cell lines, suggests an inverse association of p75 NGF-R expression with the neoplastic progression of the human prostate.

¹ This research was supported by a grant from the NIH (CA50229) to D.D.

² To whom requests for reprints should be addressed, at Department of Anatomy and Cell Biology, Georgetown University School of Medicine, 3900 Reservoir Road, N.W., Washington, DC 20007.

Received 7/ 7/92. Accepted 8/18/92.

This article has been cited by other articles:

				H. Jin, Y. Pan, L. He, H. Zhai, X. Li, L. Zhao, L. Sun, J. Liu, L. Hong, J. Song, et al. p75 Neurotrophin Receptor Inhibits Invasion and Metastasis of Gastric Cancer Mol. Cancer Res., May 1, 2007; 5(5): 423 - 433. [Abstract] [Full Text] [PDF]

				T. Okumura, S. Tsunoda, Y. Mori, T. Ito, K. Kikuchi, T. C. Wang, S. Yasumoto, and Y. Shimada The Biological Role of the Low-Affinity p75 Neurotrophin Receptor in Esophageal Squamous Cell Carcinoma Clin. Cancer Res., September 1, 2006; 12(17): 5096 - 5103. [Abstract] [Full Text] [PDF]

				D. E. BREDESEN, P. MEHLEN, and S. RABIZADEH Apoptosis and Dependence Receptors: A Molecular Basis for Cellular Addiction Physiol Rev, April 1, 2004; 84(2): 411 - 430. [Abstract] [Full Text] [PDF]

				S. J. Miknyoczki, W. Wan, H. Chang, P. Dobrzanski, B. A. Ruggeri, C. A. Dionne, and K. Buchkovich The Neurotrophin-Trk Receptor Axes Are Critical for the Growth and Progression of Human Prostatic Carcinoma and Pancreatic Ductal Adenocarcinoma Xenografts in Nude Mice Clin. Cancer Res., June 1, 2002; 8(6): 1924 - 1931. [Abstract] [Full Text] [PDF]

				C. Fiorentini, N. Guerra, M. Facchetti, A. Finardi, L. Tiberio, L. Schiaffonati, P. Spano, and C. Missale Nerve Growth Factor Regulates Dopamine D2 Receptor Expression in Prolactinoma Cell Lines via p75NGFR-Mediated Activation of Nuclear Factor-{kappa}B Mol. Endocrinol., February 1, 2002; 16(2): 353 - 366. [Abstract] [Full Text] [PDF]

				B. Davidson, P. Lazarovici, A. Ezersky, J. M. Nesland, A. Berner, B. Risberg, C. G. Trope, G. B. Kristensen, M. Goscinski, G. van de Putte, et al. Expression Levels of the Nerve Growth Factor Receptors TrkA and p75 in Effusions and Solid Tumors of Serous Ovarian Carcinoma Patients Clin. Cancer Res., November 1, 2001; 7(11): 3457 - 3464. [Abstract] [Full Text] [PDF]

				A. Ricci, S. Greco, S. Mariotta, L. Felici, E. Bronzetti, A. Cavazzana, G. Cardillo, F. Amenta, A. Bisetti, and G. Barbolini Neurotrophins and Neurotrophin Receptors in Human Lung Cancer Am. J. Respir. Cell Mol. Biol., October 1, 2001; 25(4): 439 - 446. [Abstract] [Full Text] [PDF]

				A. Chiarenza, P. Lazarovici, L. Lempereur, G. Cantarella, A. Bianchi, and R. Bernardini Tamoxifen Inhibits Nerve Growth Factor-induced Proliferation of the Human Breast Cancerous Cell Line MCF-7 Cancer Res., April 1, 2001; 61(7): 3002 - 3008. [Abstract] [Full Text]

				M. A. Sortino, F. Condorelli, C. Vancheri, A. Chiarenza, R. Bernardini, U. Consoli, and P. L. Canonico Mitogenic Effect of Nerve Growth Factor (NGF) in LNCaP Prostate Adenocarcinoma Cells: Role of the High- and Low-Affinity NGF Receptors Mol. Endocrinol., January 1, 2000; 14(1): 124 - 136. [Abstract] [Full Text]

				D. J. George, C. A. Dionne, J. Jani, T. Angeles, C. Murakata, J. Lamb, and J. T. Isaacs Sustained in Vivo Regression of Dunning H Rat Prostate Cancers Treated with Combinations of Androgen Ablation and Trk Tyrosine Kinase Inhibitors, CEP-751 (KT-6587) or CEP-701 (KT-5555) Cancer Res., May 1, 1999; 59(10): 2395 - 2401. [Abstract] [Full Text] [PDF]

				S. Sigala, I. Faraoni, D. Botticini, M. Paez-Pereda, C. Missale, E. Bonmassar, and P. Spano Suppression of Telomerase, Reexpression of KAI1, and Abrogation of Tumorigenicity by Nerve Growth Factor in Prostate Cancer Cell Lines Clin. Cancer Res., May 1, 1999; 5(5): 1211 - 1218. [Abstract] [Full Text] [PDF]

				S. Descamps, X. Lebourhis, M. Delehedde, B. Boilly, and H. Hondermarck Nerve Growth Factor Is Mitogenic for Cancerous but Not Normal Human Breast Epithelial Cells J. Biol. Chem., July 3, 1998; 273(27): 16659 - 16662. [Abstract] [Full Text] [PDF]

				P. J. Russell, S. Bennett, and P. Stricker Growth factor involvement in progression of prostate cancer Clin. Chem., April 1, 1998; 44(4): 705 - 723. [Abstract] [Full Text] [PDF]

				D. E. Bredesen REVIEW {blacksquare} : Keeping Neurons Alive: The Molecular Control of Apoptosis (Part I Neuroscientist, May 1, 1996; 2(3): 181 - 190. [Abstract] [PDF]