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A General Transcription Initiation Factor, Human Transcription Factor IID, Overexpressed in Human Lung and Breast Carcinoma and Rapidly Induced with Serum Stimulation¹

Departments of Clinical Pathology [C. W., H. O.] and Pathology [K. K., T. K.], School of Medicine, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228, Japan

A general transcription factor IID which binds to the TATA box promoter element on RNA polymerase II genes regulates and initiates eukaryotic mRNA synthesis. A quantitative polymerase chain reaction procedure was developed and the human transcription factor IID (hTFIID) transcript was measured in normal human tissues, lung carcinomas, lung carcinoma cell lines, and breast carcinomas. In some normal tissues such as liver, fetal lung, and placenta, relatively low to moderate levels of hTFIID mRNA were detected. In contrast, hTFIID transcript was highly expressed in nearly all solid lung carcinomas and cell lines including both small cell lung cancer and non-small cell lung cancer. hTFIID mRNA was present to a greater extent in small cell lung cancer than non-small cell lung cancer in solid tumors and cell lines. In solid carcinomas of breast, overexpression of hTFIID was also detected. A serum induction study using a serum-starved small cell lung cancer cell line, Lu134BS, indicated hTFIID transcription to be rapidly induced at 15 min following stimulation and its response essentially similar to that of protooncogene, c-fos. These results indicate the involvement of the expression of the general transcription factor hTFIID in lung and breast carcinoma, such as being associated with poor differentiation and high mitotic activity.

¹ This study was supported in part by Grant-in-Aid for the Encouragement of Young Scientists from the Ministry of Education, Science and Culture and Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare of Japan.

² To whom requests for reprints should be addressed.

Received 3/ 4/91. Accepted 10/30/91.

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