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Complementary DNA Cloning, Messenger RNA Expression, and Induction of -Class Glutathione S-Transferases in Mouse Tissues¹

Department of Environmental Health and Institute for Environmental Studies, University of Washington, Seattle, Washington, 98195

Glutathione S-transferases (EC 2.5.1.18) are a multigene family of related proteins divided into four classes. Each class has multiple isoforms that exhibit tissue-specific expression, which may be an important determinant of susceptibility of that tissue to toxic injury or cancer. Recent studies have suggested that -class glutathione S-transferase isoforms may play an important role in the development of cancers. Several -class glutathione S-transferase isozymes have been characterized, purified, and cloned from a number of species, including rats, mice, and humans. Here we report on the cloning, sequencing, and mRNA expression of two -class glutathione S-transferases from mouse liver, termed mYa and mYc. While mYa was shown to be identical to the known -class glutathione S-transferase complementary DNA clone pGT41 (W. R. Pearson et al., J. Biol. Chem., 263: 13324–13332, 1988), the other clone, mYc, was demonstrated to be a novel complementary DNA clone encoding a glutathione S-transferase homologous to rat Yc (subunit 2). The mRNA for this novel complementary DNA is expressed constitutively in mouse liver. It also is the major -class glutathione S-transferase isoform expressed in lung. The levels of expression of the butylated hydroxyanisole-inducible form (mYa) are highest in kidney and intestine. Treatment of mice with butylated hydroxyanisole had little effect on the expression levels of mYc but strongly induced mYa expression in liver. Butylated hydroxyanisole treatment increased expression levels for both mYa and mYc to varying degrees in kidney, lung, and intestine. The importance of the novel mouse liver -class glutathione S-transferase isoform (mYc) in the metabolism of aflatoxin B₁ and other carcinogens is discussed.

¹ This work was supported by NIH Grants ES05780, CA47561, and ES03933.

² To whom requests for reprints should be addressed, at Department of Environmental Health, SC-34, and Institute for Environmental Studies, University of Washington, Seattle, WA 98195.

Received 8/ 5/91. Accepted 10/31/91.

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