This Article Full Text (PDF) Erratum (v52,p1382) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stromberg, K. Articles by Johnson, G. R. Search for Related Content PubMed PubMed Citation Articles by Stromberg, K. Articles by Johnson, G. R.

Transforming Growth Factor- Acts as an Autocrine Growth Factor in Ovarian Carcinoma Cell Lines

Laboratory of Cell Biology, Division of Cytokine Biology, Food and Drug Administration, Bethesda, Maryland 20892

The potential of transforming growth factor- (TGF-) to function as an autocrine growth factor was evaluated in numerous ovarian carcinoma cell lines. All 17 lines which were examined expressed the epidermal growth factor receptor and 16 cell lines, in addition, concomitantly secreted TGF-. Radioimmunoassay of processed serum-free-conditioned medium indicated TGF- concentrations ranging from 16 to 197 pg/ml, or 1.5 to 95 ng/10⁸ cells. ¹²⁵I-TGF- bound to a single class of high-affinity-binding sites on the surface of the cells. The dissociation constant for the ¹²⁵I-TGF-/epidermal growth factor receptor complex ranged from 0.21 to 5.3 nM with receptor numbers from 3,500 to 96,000/cell, depending upon the cell line. The growth of 8 ovarian cell lines was stimulated in a dose-dependent manner when grown in the presence of exogenous TGF-. Growth in 4 of 5 cell lines capable of serum-free propagation was inhibited from 28 to 56% when cultured in medium containing a TGF--neutralizing monoclonal antibody. These results support the view that TGF- is an autocrine growth factor for cell lines derived from ovarian cancers of epithelial origin and suggest a potential role for TGF- in the pathogenesis or progression of the disease.

¹ To whom requests for reprints should be addressed, at the Food and Drug Administration, HFB-830, Building 29A, Room 2B-08, 8800 Rockville Pike, Bethesda, MD 20892.

Received 7/ 8/91. Accepted 10/31/91.

This article has been cited by other articles:

				S. L. Poon, G. T. Hammond, and P. C. K. Leung Epidermal Growth Factor-Induced GnRH-II Synthesis Contributes to Ovarian Cancer Cell Invasion Mol. Endocrinol., October 1, 2009; 23(10): 1646 - 1656. [Abstract] [Full Text] [PDF]

				J.-H. Choi, K.-C. Choi, N. Auersperg, and P. C K Leung Gonadotropins upregulate the epidermal growth factor receptor through activation of mitogen-activated protein kinases and phosphatidyl-inositol-3-kinase in human ovarian surface epithelial cells Endocr. Relat. Cancer, June 1, 2005; 12(2): 407 - 421. [Abstract] [Full Text] [PDF]

				D. Milliken, C. Scotton, S. Raju, F. Balkwill, and J. Wilson Analysis of Chemokines and Chemokine Receptor Expression in Ovarian Cancer Ascites Clin. Cancer Res., April 1, 2002; 8(4): 1108 - 1114. [Abstract] [Full Text] [PDF]

				O. Alper, E. S. Bergmann-Leitner, T. A. Bennett, N. F. Hacker, K. Stromberg, and W. G. Stetler-Stevenson Epidermal Growth Factor Receptor Signaling and the Invasive Phenotype of Ovarian Carcinoma Cells J Natl Cancer Inst, September 19, 2001; 93(18): 1375 - 1384. [Abstract] [Full Text] [PDF]

				V. Doraiswamy, J. A. Parrott, and M. K. Skinner Expression and Action of Transforming Growth Factor Alpha in Normal Ovarian Surface Epithelium and Ovarian Cancer Biol Reprod, September 1, 2000; 63(3): 789 - 796. [Abstract] [Full Text]

				A. Bagnato, D. Salani, V. Di Castro, J. R. Wu-Wong, R. Tecce, M. R. Nicotra, A. Venuti, and P. G. Natali Expression of Endothelin 1 and Endothelin A Receptor in Ovarian Carcinoma: Evidence for an Autocrine Role in Tumor Growth Cancer Res., February 1, 1999; 59(3): 720 - 727. [Abstract] [Full Text] [PDF]

				D. Coppola, E. Catalano, and S. V. Nicosia Transforming Growth Factor-alpha and c-erbB-2 Oncogene Products in Ovarian Epithelial Tumors International Journal of Surgical Pathology, January 1, 1996; 4(3): 129 - 135. [Abstract] [PDF]

				A. Berchuck, A. Elbendary, L. Havrilesky, G. C. Rodriguez, and R. C. Bast Pathogenesis of Ovarian Cancers Reproductive Sciences, July 1, 1994; 1(3): 181 - 190. [Abstract] [PDF]

				K. Rosen, M. L. Coll, A. Li, and J. Filmus Transforming Growth Factor-alpha Prevents Detachment-induced Inhibition of c-Src Kinase Activity, Bcl-XL Down-regulation, and Apoptosis of Intestinal Epithelial Cells J. Biol. Chem., September 28, 2001; 276(40): 37273 - 37279. [Abstract] [Full Text] [PDF]