This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Anglard, P. Articles by Linehan, W. M. Search for Related Content PubMed PubMed Citation Articles by Anglard, P. Articles by Linehan, W. M.

Molecular and Cellular Characterization of Human Renal Cell Carcinoma Cell Lines

Urologic Oncology Section, Surgery Branch, Division of Cancer Treatment [P. A., E. T., S. L., M. L.] and Laboratory of Pathology [M. M.], National Cancer Institute, Bethesda, Maryland 20892, and the Laboratory of Immunobiology, National Cancer Institute-Frederick Cancer Research Facility [F. L., M. L., B. Z.], Division of Cancer Biology and Diagnosis, National Cancer Institute, Frederick, Maryland 21701

Recent studies have suggested that a tumor suppressor gene located in the region 3p21–26 of chromosome 3 is essential to the genesis of sporadic renal cell carcinoma (RCC) and that other tumor suppressor genes located on other chromosomes may be involved with progression of this malignancy.

The cellular heterogeneity of solid tumors complicates their analysis. In order to analyze a homogeneous population of tumor cells and identify genetic changes associated with histology in renal cortical tumors, we have established and characterized 35 RCC lines derived from tumor tissue from 31 patients with renal cell carcinomas. The overall success rate in establishing these cell lines from fresh or frozen specimens was 75% (18 of 24) and 35% (17 of 48), respectively. These lines differed in their morphology, growth rates, and tumorigenicity in athymic nude mice. Molecular characterization utilizing DNA fingerprinting and restriction fragment length polymorphism deletion analysis was performed to detect somatic mutations and loss of heterozygosity on the short arm of chromosome 3. Analysis revealed loss of heterozygosity on chromosome 3p in 25 cell lines derived from 28 informative nonpapillary forms of RCC (89%). Deletion-mapping analysis showed the retention of the distal locus, D3S18, in one of the RCC cell lines, which further localized the position of the putative tumor suppressor gene to the region proximal to D3S18. Although deletions on chromosome 3 have been recently suggested to be specific to the clear cell-type phenotype, our results revealed no correlation between loss of heterozygosity and clear or granular cell types.

¹ Present address: Laboratoire de génétique moléculaire des eucaryotes du CNRS, Unité 184 de biologie moléculaire et de génie génétique de l'INSERM, Institut de Chimie Biologique, Faculté de médecine, 11 rue Humann, 67085 Strasbourg Cédex, France.

² To whom requests for reprints should be addressed, at Urologic Oncology Section, Surgery Branch, National Cancer Institute, Bldg. 10, Room 2B47, Bethesda, MD 20892.

Received 7/30/91. Accepted 11/ 1/91.

This article has been cited by other articles:

				C. Sourbier, S. Danilin, V. Lindner, J. Steger, S. Rothhut, N. Meyer, D. Jacqmin, J.-J. Helwig, H. Lang, and T. Massfelder Targeting the Nuclear Factor-{kappa}B Rescue Pathway Has Promising Future in Human Renal Cell Carcinoma Therapy Cancer Res., December 15, 2007; 67(24): 11668 - 11676. [Abstract] [Full Text] [PDF]

				C. Sourbier, V. Lindner, H. Lang, A. Agouni, E. Schordan, S. Danilin, S. Rothhut, D. Jacqmin, J.-J. Helwig, and T. Massfelder The Phosphoinositide 3-Kinase/Akt Pathway: A New Target in Human Renal Cell Carcinoma Therapy. Cancer Res., May 15, 2006; 66(10): 5130 - 5142. [Abstract] [Full Text] [PDF]

				I. Talon, V. Lindner, C. Sourbier, E. Schordan, S. Rothhut, M. Barthelmebs, H. Lang, J.-J. Helwig, and T. Massfelder Antitumor effect of parathyroid hormone-related protein neutralizing antibody in human renal cell carcinoma in vitro and in vivo Carcinogenesis, January 1, 2006; 27(1): 73 - 83. [Abstract] [Full Text] [PDF]

				T. Massfelder, H. Lang, E. Schordan, V. Lindner, S. Rothhut, S. Welsch, P. Simon-Assmann, M. Barthelmebs, D. Jacqmin, and J.-J. Helwig Parathyroid Hormone-Related Protein Is an Essential Growth Factor for Human Clear Cell Renal Carcinoma and a Target for the von Hippel-Lindau Tumor Suppressor Gene Cancer Res., January 1, 2004; 64(1): 180 - 188. [Abstract] [Full Text] [PDF]

				I. Y. Kim, D.-H. Lee, D. K. Lee, B. C. Kim, H. T. Kim, F. S. Leach, W. M. Linehan, R. A. Morton, and S. J. Kim Decreased Expression of Bone Morphogenetic Protein (BMP) Receptor Type II Correlates with Insensitivity to BMP-6 in Human Renal Cell Carcinoma Cells Clin. Cancer Res., December 1, 2003; 9(16): 6046 - 6051. [Abstract] [Full Text] [PDF]

				M. I. Nishimura, D. Avichezer, M. C. Custer, C. S. Lee, C. Chen, M. R. Parkhurst, R. A. Diamond, P. F. Robbins, D. J. Schwartzentruber, and S. A. Rosenberg MHC Class I-restricted Recognition of a Melanoma Antigen by a Human CD4+ Tumor Infiltrating Lymphocyte Cancer Res., December 1, 1999; 59(24): 6230 - 6238. [Abstract] [Full Text] [PDF]

				M. S. LIPKOWITZ, B. HANSS, N. TULCHIN, P. D. WILSON, J. C. LANGER, M. D. ROSS, G. J. KURTZMAN, P. E. KLOTMAN, and M. E. KLOTMAN Transduction of Renal Cells in Vitro and in Vivo by Adeno-Associated Virus Gene Therapy Vectors J. Am. Soc. Nephrol., September 1, 1999; 10(9): 1908 - 1915. [Abstract] [Full Text]

				P. Anglard, T. Melot, E. Guérin, G. Thomas, and P. Basset Structure and Promoter Characterization of the Human Stromelysin-3 Gene J. Biol. Chem., September 1, 1995; 270(35): 20337 - 20344. [Abstract] [Full Text] [PDF]