This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jansen, B. Articles by Kersey, J. H. Search for Related Content PubMed PubMed Citation Articles by Jansen, B. Articles by Kersey, J. H.

Establishment of a Human t(4;11) Leukemia in Severe Combined Immunodeficient Mice and Successful Treatment Using Anti-CD19 (B43)-Pokeweed Antiviral Protein Immunotoxin¹

Bone Marrow Transplantation Program [F. M. U., J. H. K.] and Departments of Laboratory Medicine/Pathology [B. J., W. B. J., J. H. K.], Pediatrics [F. M. U., J. H. K.], and Therapeutic Radiology [F. M. U.], University of Minnesota, Minneapolis, Minnesota 55455

Human acute leukemia, with a chromosomal translocation involving chromosomes 4 and 11, t(4;11)(q21;q23), is the most common form of leukemia in infants and responds very poorly to conventional therapy. A human CD19⁺ mixed-lineage leukemia cell line with a t(4;11)(q21;q23) translocation, RS4;11, disseminated and proliferated in the hematopoietic tissues and other organs of mice with severe combined immunodeficiency in a manner similar to that observed in humans and killed 100% of the animals. The anti-CD19(B43)-pokeweed antiviral protein immunotoxin selectively inhibited clonogenic RS4;11 cells in vitro, markedly reduced the burden of disseminated leukemia of severe combined immunodeficient mice, and, most importantly, resulted in the long-term survival of treated animals. This severe combined immunodeficient mouse model should be useful for the design of more effective treatment strategies for refractory human leukemias.

¹ This work was supported by Grants CA49721, CA21737, and CA51425 from the National Cancer Institute/NIH and the Childrens Cancer Research Fund. J. H. K. is the recipient of an Outstanding Investigator Award (CA49721) from the National Cancer Institute. F. M. U. is a Scholar of the Leukemia Society of America.

² To whom requests for reprints should be addressed, at University of Minnesota, Department of Pediatrics, Box 86 UMHC, 420 Delaware Street, Minneapolis, MN 55455.

Received 7/18/91. Accepted 11/ 1/91.

This article has been cited by other articles:

				L. Qi, T. M. Nett, M. C. Allen, X. Sha, G. S. Harrison, B. A. Frederick, E. D. Crawford, and L. M. Glode Binding and Cytotoxicity of Conjugated and Recombinant Fusion Proteins Targeted to the Gonadotropin-Releasing Hormone Receptor Cancer Res., March 15, 2004; 64(6): 2090 - 2095. [Abstract] [Full Text] [PDF]

				W.-H. Yang, M. Wieczorck, M. C. Allen, and T. M. Nett Cytotoxic Activity of Gonadotropin-Releasing Hormone (GnRH)-Pokeweed Antiviral Protein Conjugates in Cell Lines Expressing GnRH Receptors Endocrinology, April 1, 2003; 144(4): 1456 - 1463. [Abstract] [Full Text] [PDF]

				H Ali, M Loizidou, M Dashwood, F Savage, C Sheard, and I Taylor Stimulation of colorectal cancer cell line growth by ET-1 and its inhibition by ETA antagonists Gut, November 1, 2000; 47(5): 685 - 688. [Abstract] [Full Text] [PDF]