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Increased Inosine-5'-phosphate Dehydrogenase Gene Expression in Solid Tumor Tissues and Tumor Cell Lines¹

Biological and Medical Research Division, Argonne National Laboratory, Argonne, Illinois 60439 [F. R. C., C. B. C., E. H.], and Northwestern University Medical School, The Children's Memorial Institute for Education and Research, Chicago, Illinois 60614 [B. L. M.]

Inosine-5'-phosphate (IMP) dehydrogenase, a regulatory enzyme of guanine nucleotide biosynthesis, may play a role in cell proliferation and malignancy. To assess this role we examined IMP dehydrogenase expression in a series of human solid tumor tissues and tumor cell lines in comparison with their normal counterparts. Increased IMP dehydrogenase gene expression was observed in brain tumors relative to normal brain tissue and in sarcoma cells relative to normal fibroblasts. Similarly, in several B- and T-lymphoid leukemia cell lines, elevated levels of IMP dehydrogenase mRNA and cellular enzyme were observed in comparison with the levels in peripheral blood lymphocytes. These results are consistent with an association between increased IMP dehydrogenase expression and either enhanced cell proliferation or malignant transformation.

¹ This work was supported by the United States Department of Energy, Office of Health and Environmental Research under Contract W-31-109-ENG-38.

Received 7/20/92. Accepted 9/ 1/92.

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