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Tumor Cell Surface-associated Binding Site for the M_r 72,000 Type IV Collagenase¹

Laboratory of Biology, University of Liege, Belgium [H. P. E., A. G. R., A. C. N., J.-M. F.]; CNRS, URA 1459, Institut Pasteur, Lyon, France [H. P. E., J-A. G.]; and Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, MD [W. G. S-S.]

We have studied the capacity of two human breast adenocarcinoma cells, MDA-MB231 and MCF-7, to bind exogenous M_r 72,000 type IV collagenase by both morphological and radioreceptor binding assays. By indirect immunofluorescence, staining with a specific anti-M_r 72,000 type IV collagenase antibody was strongly induced when cells were preincubated with the purified enzyme. Scatchard plot analysis indicated the existence of a binding site for the M_r 72,000 type IV collagenase with high affinity for both cell lines (K_d = 2 x 10^-9 M). These results are the first demonstration of the existence of a tumor cell membrane-associated putative receptor for a member of the matrix metalloproteinase family, as previously evidenced for the urokinase-type plasminogen activator.

¹ This study was supported by Grant 9.4539.87 of the "Loterie Nationale" in Belgium; Grant 3.4510.90 of the FRSM in Belgium; and grants from the "Fonds Cancérologique de la CGER," the "Association Sportive contre le Cancer" (Belgium), the Faculty of Medicine (University of Liege, Belgium), the "Centre Anticancéreux prés l'Université de Liège," and the "Communauté Française de Belgique" ("Action de Recherche Concertée"; Grant 90/94-139).

² To whom requests for reprints should be addressed, at the Laboratory of Biology, University of Liege, Tower of Pathology (B23), Sart Tilman, B 4000 Liege, Belgium.

Received 5/ 8/92. Accepted 9/10/92.

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