This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weidmann, E. Articles by Whiteside, T. L. Search for Related Content PubMed PubMed Citation Articles by Weidmann, E. Articles by Whiteside, T. L.

Receptors for Interleukin 2 on Human Squamous Cell Carcinoma Cell Lines and Tumor in Situ¹

Pittsburgh Cancer Institute [E. W., M. S., D. S. H., S. Y., W-c. L., J. T. J., R. B. H., T. L. W.]; the Departments of Otolaryngology [M. S., D. S. H., J. T. J.], Pathology [R. B. H., T. L. W.], and Medicine [R. B. H.], University of Pittsburgh School of Medicine; Eye and Ear Institute [S. P., J. T. J.], Pittsburgh, Pennsylvania 15213; and INSERM U-268, Hôpital Paul-Brousse, 94800 Villejuif, France [B. A.]

Several human head and neck squamous carcinoma cell lines were found to bind ¹²⁵I-labeled or fluorescein-labeled interleukin 2 (IL-2). This binding was inhibited by an excess of cold ligand, IL-2, and by anti-p55 and anti-p70 monoclonal antibodies to the and ß chains, respectively, of the IL-2 receptor (IL-2R). A small number (300/cell) of high-affinity IL-2R (2 x 10^-12 M) and a larger number (>13,000/cells) of intermediate-affinity IL-2R (3 x 10^-10 M) were present on these tumor cells. By affinity cross-linking, tumor cells were shown to bind ¹²⁵I-IL-2 to a M_r 66,000 and 55,000 doublet peptide. The and ß chains of the IL-2R also were detected on the surface of cultured tumor cells using the relevant monoclonal antibodies and flow cytometry. Immunoperoxidase staining with anti-p70 monoclonal antibody confirmed the expression of IL-2R on squamous cell carcinomas of the head and neck in situ. The presence of transcripts for p55/IL-2R- and p70/IL-2R-ß in PCI-1 cells was confirmed by the polymerase chain reaction followed by hybridization to the IL-2R- complementary DNA probe or IL-2R-ß complementary DNA probe, respectively. Our observations demonstrate that intermediate-affinity and high-affinity IL-2Rs are expressed on some human squamous cell carcinomas of the head and neck and that the receptors are functional, because growth of these tumor cell lines can be directly inhibited by exogenously supplied IL-2. The presence of IL-2R on human solid tumors could be important to consider, in addition to immunomodulatory effects of IL-2, in developing optimal therapeutic strategies for the administration of IL-2 to patients with cancer.

¹ Supported in part by American Cancer Society Grants IM27077 and IM588A to T. L. W. and the Mary Hillman Jennings Foundation (J. T. J.). E. W. was supported by Deutche Forschungsgemeinschaft (Grant We 13351-1).

² To whom requests for reprints should be addressed, at Pittsburgh Cancer Institute, Room W1041, Biomedical Science Tower, DeSoto at O'Hara Street, Pittsburgh, PA 15213.

Received 4/ 9/92. Accepted 8/25/92.

This article has been cited by other articles:

				S. Hoving, F. Brunstein, G. aan de Wiel-Ambagtsheer, S. T. van Tiel, G. de Boeck, E. A. de Bruijn, A. M.M. Eggermont, and T. L.M. ten Hagen Synergistic Antitumor Response of Interleukin 2 with Melphalan in Isolated Limb Perfusion in Soft Tissue Sarcoma-Bearing Rats Cancer Res., May 15, 2005; 65(10): 4300 - 4308. [Abstract] [Full Text] [PDF]

				C. Quan and S.-J. Lu Identification of genes preferentially expressed in mammary epithelial cells of Copenhagen rat using subtractive hybridization and microarrays Carcinogenesis, October 1, 2003; 24(10): 1593 - 1599. [Abstract] [Full Text] [PDF]

				L.-S. Wang, K.-C. Chow, W.-Y. Li, C.-C. Liu, Y.-C. Wu, and M.-H. Huang Clinical Significance of Serum Soluble Interleukin 2 Receptor-{{alpha}} in Esophageal Squamous Cell Carcinoma Clin. Cancer Res., April 1, 2000; 6(4): 1445 - 1451. [Abstract] [Full Text]

				C A Maxwell-Armstrong, L G Durrant, R A Robins, A M Galvin, J H Scholefield, and J D Hardcastle Increased activation of lymphocytes infiltrating primary colorectal cancers following immunisation with the anti-idiotypic monoclonal antibody 105AD7 Gut, October 1, 1999; 45(4): 593 - 598. [Abstract] [Full Text] [PDF]

				M. R. Posner, L. A. Cavacini, M. P. Upton, K. C. Tillman, E. R. Gornstein, and C. M. Norris Jr. Surface Membrane-expressed CD40 Is Present on Tumor Cells from Squamous Cell Cancer of the Head and Neck in Vitro and in Vivo and Regulates Cell Growth in Tumor Cell Lines Clin. Cancer Res., August 1, 1999; 5(8): 2261 - 2270. [Abstract] [Full Text] [PDF]

				T. E. Reichert, S. Watkins, J. Stanson, J. T. Johnson, and T. L. Whiteside Endogenous IL-2 in Cancer Cells: A Marker of Cellular Proliferation J. Histochem. Cytochem., May 1, 1998; 46(5): 603 - 612. [Abstract] [Full Text]

				M. R. Hassuneh, P. S. Nagarkatti, and M. Nagarkatti Evidence for the Participation of Interleukin-2 (IL-2) and IL-4 in the Regulation of Autonomous Growth and Tumorigenesis of Transformed Cells of Lymphoid Origin Blood, January 15, 1997; 89(2): 610 - 620. [Abstract] [Full Text] [PDF]

				V. N. Tsytsikov, V. V. Yurovsky, S. P. Atamas, W. J. Alms, and B. White Identification and Characterization of Two Alternative Splice Variants of Human Interleukin-2 J. Biol. Chem., September 20, 1996; 271(38): 23055 - 23060. [Abstract] [Full Text] [PDF]