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Binding of Human Single-Stranded DNA Binding Protein to DNA Damaged by the Anticancer Drug cis-Diamminedichloroplatinum (II)¹

CRC Department of Medical Oncology, CRC Beatson Laboratories, Switchback Road, Glasgow G61 1BD [C. K. C., K. M., R. B.], and CRC Laboratories, Department of Biochemistry, The University of Dundee, Dundee DD1 4HN [M. K. K.], Scotland

The chemotherapeutic drug cis-diamminedichloroplatinum (II) covalently binds to DNA resulting in a variety of adducts and cross-links which are thought to be responsible for the toxicity of the drug. We have used the gel mobility shift assay to detect proteins which bind to DNA treated in vitro with cis-diamminedichloroplatinum (II) and have identified two complexes which bind with increased affinity to cis-diamminedichloroplatinum (II)-damaged DNA. Using monoclonal antibodies we have shown that one complex, B1, contains human single-stranded DNA binding protein, a protein known to be involved in the in vitro repair synthesis assay of mammalian excision repair.

¹ This work was supported by the Cancer Research Campaign.

² To whom requests for reprints should be addressed.

Received 7/11/92. Accepted 10/ 1/92.

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