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Frequent Somatic Mutations of the APC Gene in Human Pancreatic Cancer¹

Department of Biochemistry [A. H., S. N., Y. M., S. I., H. N., H. A., Y. N.] and Pathology [A. Y., E. T., Y. K.], Cancer Institute, 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170, Japan

² To whom requests for reprints should be addressed.

The APC (adenomatous polyposis coli) gene is responsible for familial adenomatous polyposis and is also associated with the development of sporadic tumors of the colon and stomach. To investigate whether or not mutations of APC play any role in tumors arising in other organs, we examined somatic mutations of this gene in sporadic (nonfamilial) renal cell carcinomas, hepatocellular carcinomas, and cancers of the lung and pancreas. DNAs isolated from tumors were examined by means of a RNase protection analysis, coupled with the polymerase chain reaction followed by DNA sequencing of the polymerase chain reaction products. By screening a part of the APC coding region, we detected somatic mutations in four of ten pancreatic cancers; each of these mutations would yield a truncated APC product due to a 1- or S-base pair deletion. These results imply that mutations in APC contribute to carcinogenesis in the pancreas.

¹ This work was supported in part by the Ministry of Education, Culture and Science of Japan and in part by the Vehicle Racing Commemorative Foundation.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 8/24/92. Accepted 10/13/92.

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