This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Reuvekamp, P. T. W. Articles by Buys, C. H. C. M. Search for Related Content PubMed PubMed Citation Articles by Reuvekamp, P. T. W. Articles by Buys, C. H. C. M.

Analysis of a Metastasizing Testicular Mixed Gonadal Stromal Tumor with Osteosarcoma Components Suggests That a Malignant Tumor with the Histology of Osteosarcoma May Develop without Primary Involvement of RB1 and TP53¹

Department of Medical Genetics, University of Groningen, Antonius Deusinglaan 4, 9713 AW Groningen [P. T. W. R., R. P. S., H. S., C. H. C. M. B.]; Department of Surgical Oncology, University Hospital of Groningen, Oostersingel 59, 9713 EZ Groningen[H. S. K.]; and Dr. Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam [J. W. O.], the Netherlands

² To whom requests for reprints should be addressed, at Department of Medical Genetics, Antonius Deusinglaan 4, 9713 AW Groningen, the Netherlands.

A malignant stromal tumor of the testis with an osteosarcoma component and five of its metastases mainly containing osteosarcoma have been analyzed for RB1 and TP53 abnormalities. Whereas in the primary tumor and in some of the metastases loss of heterozygosity could not be detected for RB1 or for the 17p13 region in which TP53 is located, other metastases showed such losses of heterozygosity. By polymerase chain reaction analysis an 18-base pair deletion from exon 5 of the TP53 gene was found in a small proportion of primary tumor cells and in one of the metastases, but not in the other metastases. Therefore, in this case neither RB1 nor TPS3 seems to play an essential role in the initiation of osteosarcoma.

¹ This work was supported by the Dutch Cancer Society.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 7/27/92. Accepted 10/12/92.