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High Molecular Weight Transforming Growth Factor β Is Excreted in the Urine in Active Nodular Sclerosing Hodgkin's Disease¹

Department of Medicine, Division of Hematology/Oncology, Emory University School of Medicine, Atlanta, Georgia 30327

² To whom requests for reprints should be addressed, at Emory University, Woodruff Research Building, 46 Armstrong St., Atlanta, GA 30303.

To measure the in vivo secretion of high molecular weight (HMW) transforming growth factor (TGF)β by Reed-Sternberg cells from patients with nodular sclerosing Hodgkin's disease, we studied the urine samples from untreated patients. The urinary proteins did not promote the proliferation of NIH-3T3 cells in monolayer culture and contained similar amounts of total TGF activity when compared with normal controls. Urinary proteins from 24 different control and test urines were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. Either of two primary antibodies were used for immunoblot detection: (a) affinity column purified polyclonal anti-TGFβ₁, prepared against platelet TGFβ₁ or (b) monoclonal anti-HMW-TGFβ prepared against HMW-TGFβ secreted by cloned L-428 Reed-Sternberg cells. All patients with active nodular sclerosing Hodgkin's disease had a detectable HMW-TGFβ (300,000) which cross-reacted with both anti-TGFβ1 and anti-HMW-TGFβ. Purification demonstrated HMW-TGFβ which was active at physiological pH. Twelve control urine samples from healthy adults and 5 follow-up samples from the Hodgkin's patients after successful treatment contained no detectable urinary HMW-TGFβ. The in vivo production of HMW-TGFβ in untreated nodular sclerosing Hodgkin's disease supports the conclusion that this growth factor is secreted in large amounts by Reed-Sternberg cells or cells stimulated by Reed-Sternberg cells.

¹ This work was supported by Grants CA 50739 and CA 30565 from the National Institutes of Health, Bethesda, MD.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 3/23/92. Accepted 10/ 5/92.

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				R. E. Brown and N. R. Kamal The Reed-Sternberg Cell: Molecular Characterization by Proteomic Analysis with Therapeutic Implications Ann. Clin. Lab. Sci., October 1, 2002; 32(4): 339 - 351. [Abstract] [Full Text] [PDF]