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Department of Pathology [H. Y., K. O., T. K.] and Experimental Pathology [O. H.] of the Cancer Institute, Tokyo, 1-37-1, Kami-ikebukuro, Toshima-ku, Tokyo 170 and the Department of Pathology [H. Y., R. M.], Faculty of Medicine, University of Tokyo School of Medicine, 7-3-1, Hongo, Bunkyo-ku Tokyo 113, Japan
2 To whom requests for reprints should be addressed.
A total of 83 cirrhotic nodules (pseudolobules) individually collected from 11 cirrhotic livers of hepatitis B virus carrier patient were analyzed for the frequency and mode of hepatitis B virus integration as well as histological features. Southern blot analysis disclosed discrete bands at higher molecular weight region in 26 of 83 nodules (31.3%), indicating a clonal growth of hepatocytes with viral integration. Considerable variation (0–75%) existed in the positive rates for discrete bands in nodules among livers. Molecular cloning revealed the sequence flanking an integrated viral sequence to be host DNA and thus confirmed true integration.
Histological analysis, however, did not reveal any neoplastic-appearing foci of growth within nodules, despite the fact that the detection sensitivity would predict clones of more than 105 cells to give rise to clonal integration patterns on Southern blot analysis. The question of whether clonal expansion of hepatocytes reflects any viral integration-associated growth advantage and/or a preneoplastic condition awaits future studies.
1 This work was sponsored by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Received 9/18/92. Accepted 10/ 7/92.
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