This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Economos, K. Articles by Casey, M. L. Search for Related Content PubMed PubMed Citation Articles by Economos, K. Articles by Casey, M. L.

Endothelin-1 Gene Expression and Biosynthesis in Human Endometrial HEC-1A Cancer Cells¹

The Cecil H. and Ida Green Center for Reproductive Biology Sciences [K. E., P. C. M., M. L. C.] and the Departments of Obstetrics and Gynecology [K. E., P. C. M., M. L. C.] and Biochemistry [P. C. M., M. L. C.], The University of Texas Southwestern Medical Center, Dallas, Texas 75235-9051

In this study, evidence was obtained that endothelin-1 (ET-1) is produced by an established endometrial cancer (HEC-1A) cell line. PreproET-1 mRNA is present in HEC-1A cells, and immunoreactive endothelin is secreted into the medium of these cells maintained in culture. Cycloheximide treatment of these cells caused superinduction of preproET-1 mRNA. Transforming growth factor-ß acts in these cells to increase the levels of preproET-1 mRNA. This effect of transforming growth factor-ß on preproET-1 mRNA accumulation was accompanied by an increase in the amount of immunoreactive endothelin secreted into the culture medium. ET-1, added to the culture medium, did not act as a mitogen in HEC-1A cells. We speculate that ET-1 (which is known to stimulate fibroblast proliferation) produced by endometrial adenocarcinoma cells may participate in the angiogenic process that occurs during the establishment of this carcinoma in vivo.

¹ This investigation was supported, in part, by USPHS Grant 5-P50-HD11149.

² To whom requests for reprints should be addressed, at The Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235-9051.

Received 8/ 9/91. Accepted 11/11/91.

This article has been cited by other articles:

				W. H. Moretz III, C. G. Gourin, D. J. Terris, Z.-S. Xia, Z. Liu, P. M. Weinberger, E. Chin, and B.-L. Adam Detection of Papillary Thyroid Carcinoma With Serum Protein Profile Analysis Arch Otolaryngol Head Neck Surg, February 1, 2008; 134(2): 198 - 202. [Abstract] [Full Text] [PDF]

				A. A. Kazi, J. M. Jones, and R. D. Koos Chromatin Immunoprecipitation Analysis of Gene Expression in the Rat Uterus in Vivo: Estrogen-Induced Recruitment of Both Estrogen Receptor {alpha} and Hypoxia-Inducible Factor 1 to the Vascular Endothelial Growth Factor Promoter Mol. Endocrinol., August 1, 2005; 19(8): 2006 - 2019. [Abstract] [Full Text] [PDF]

				R. D. KOOS, A. A. KAZI, M. S. ROBERSON, and J. M. JONES New Insight into the Transcriptional Regulation of Vascular Endothelial Growth Factor Expression in the Endometrium by Estrogen and Relaxin Ann. N.Y. Acad. Sci., May 1, 2005; 1041(1): 233 - 247. [Abstract] [Full Text] [PDF]

				F. Spinella, L. Rosano, V. Di Castro, M. R. Nicotra, P. G. Natali, and A. Bagnato Endothelin-1 Decreases Gap Junctional Intercellular Communication by Inducing Phosphorylation of Connexin 43 in Human Ovarian Carcinoma Cells J. Biol. Chem., October 17, 2003; 278(42): 41294 - 41301. [Abstract] [Full Text] [PDF]

				G. Moreno-Bueno, C. Sanchez-Estevez, R. Cassia, S. Rodriguez-Perales, R. Diaz-Uriarte, O. Dominguez, D. Hardisson, M. Andujar, J. Prat, X. Matias-Guiu, et al. Differential Gene Expression Profile in Endometrioid and Nonendometrioid Endometrial Carcinoma: STK15 Is Frequently Overexpressed and Amplified in Nonendometrioid Carcinomas Cancer Res., September 15, 2003; 63(18): 5697 - 5702. [Abstract] [Full Text] [PDF]

				L. Rosano, F. Spinella, D. Salani, V. Di Castro, A. Venuti, M. R. Nicotra, P. G. Natali, and A. Bagnato Therapeutic Targeting of the Endothelin A Receptor in Human Ovarian Carcinoma Cancer Res., May 15, 2003; 63(10): 2447 - 2453. [Abstract] [Full Text] [PDF]

				S. I. Ahmed, J. Thompson, J. M. Coulson, and P. J. Woll Studies on the Expression of Endothelin, Its Receptor Subtypes, and Converting Enzymes in Lung Cancer and in Human Bronchial Epithelium Am. J. Respir. Cell Mol. Biol., April 1, 2000; 22(4): 422 - 431. [Abstract] [Full Text]