| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037 [T. G. C., J. M. G., D. R. G.], and Department of Biology, St. Patrick's College, Maynooth, County Kildare, Ireland [T. G. C., S. V. L., J. M. G.]
Apoptosis is a form of cell death in which the cell "participates," such that metabolic energy and often protein synthesis are required for the death to occur. Once begun, the process of apoptosis proceeds in an ordered fashion. In the earliest phase DNA fragmentation occurs, accompanied by cell shrinkage and dilation of the endoplasmic reticulum. This is followed by cell fragmentation with the formation of sealed membrane vesicles, termed apoptotic bodies. In the present study we have demonstrated that the fungal metabolite cytochalasin B inhibits cell fragmentation and the formation of apoptotic bodies, probably by its ability to interfere with actin polymerization. This effect was seen when HL-60 cells were pretreated with cytochalasin B and then exposed to one of a number of apoptosis-inducing agents, including UV irradiation, camptothecin, aphidocholin, or PMA plus ionomycin. The observed effect was not peculiar to HL-60 cells, inasmuch as it was also seen for both Molt-4 and U-937 cell lines. Cytochalasin B had no effect on DNA fragmentation occurring in the earliest stage of apoptosis, and it appeared to have no inhibitory effects on nuclear fragmentation. Staurosporin had an effect similar to that seen with cytochalasin B, probably due to its ability to inhibit protein kinase C, which is a known potentiator of microfilament assembly. These data demonstrate that microfilament assembly is necessary for the formation of apoptotic bodies in the later stages of the apoptotic process.
1 Supported by grants to D. R. G. from Gemini Science and to T. G. C. and S. V. L. from the Irish Cancer Society and The Children's Leukemia Research Project.
2 To whom requests for reprints should be addressed, at Department of Biology, St. Patrick's College, Maynooth, Co. Kildare, Ireland.
Received 9/ 9/91. Accepted 12/ 2/91.
This article has been cited by other articles:
|
|
 |
|
  C. D. Bortner, M. I. Sifre, and J. A. Cidlowski Cationic Gradient Reversal and Cytoskeleton-independent Volume Regulatory Pathways Define an Early Stage of Apoptosis J. Biol. Chem., March 14, 2008; 283(11): 7219 - 7229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Moss, V. M. Betin, S. D. Malesinski, and J. D. Lane A novel role for microtubules in apoptotic chromatin dynamics and cellular fragmentation J. Cell Sci., June 1, 2006; 119(11): 2362 - 2374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Lane, V. J. Allan, and P. G. Woodman Active relocation of chromatin and endoplasmic reticulum into blebs in late apoptotic cells J. Cell Sci., September 1, 2005; 118(17): 4059 - 4071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tomiyoshi, Y. Horita, M. Nishita, K. Ohashi, and K. Mizuno Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing Genes Cells, June 1, 2004; 9(6): 591 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. VanWijk, E. VanBavel, A. Sturk, and R. Nieuwland Microparticles in cardiovascular diseases Cardiovasc Res, August 1, 2003; 59(2): 277 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Broxmeyer, L. Kohli, C. H. Kim, Y. Lee, C. Mantel, S. Cooper, G. Hangoc, M. Shaheen, X. Li, and D. W. Clapp Stromal cell-derived factor-1/CXCL12 directly enhances survival/antiapoptosis of myeloid progenitor cells through CXCR4 and G{alpha}i proteins and enhances engraftment of competitive, repopulating stem cells J. Leukoc. Biol., May 1, 2003; 73(5): 630 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Eissner, G. Multhoff, A. Gerbitz, S. Kirchner, S. Bauer, S. Haffner, D. Sondermann, R. Andreesen, and E. Holler Fludarabine induces apoptosis, activation, and allogenicity in human endothelial and epithelial cells: protective effect of defibrotide Blood, June 17, 2002; 100(1): 334 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Comin-Anduix, N. Agell, O. Bachs, J. Ovadi, and M. Cascante A New Bis-Indole, KARs, Induces Selective M Arrest with Specific Spindle Aberration in Neuroblastoma Cell Line SH-SY5Y Mol. Pharmacol., December 1, 2001; 60(6): 1235 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Ling, Y. Zhong, and R. Perez-Soler Disruption of Cell Adhesion and Caspase-Mediated Proteolysis of {beta}- and {gamma}-Catenins and APC Protein in Paclitaxel-Induced Apoptosis Mol. Pharmacol., March 1, 2001; 59(3): 593 - 603. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Eissner, S. Kirchner, H. Lindner, W. Kolch, P. Janosch, M. Grell, P. Scheurich, R. Andreesen, and E. Holler Reverse Signaling Through Transmembrane TNF Confers Resistance to Lipopolysaccharide in Human Monocytes and Macrophages J. Immunol., June 15, 2000; 164(12): 6193 - 6198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Chamoux Involvement of the Angiotensin II Type 2 Receptor in Apoptosis during Human Fetal Adrenal Gland Development J. Clin. Endocrinol. Metab., December 1, 1999; 84(12): 4722 - 4730. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. C. Mills, N. L. Stone, and R. N. Pittman Extranuclear Apoptosis: The Role of the Cytoplasm in the Execution Phase J. Cell Biol., August 23, 1999; 146(4): 703 - 708. [Full Text] [PDF]
|
|
|
|
|
|
C. Segundo, F. Medina, C. Rodriguez, R. Martinez-Palencia, F. Leyva-Cobian, and J. A. Brieva Surface Molecule Loss and Bleb Formation by Human Germinal Center B Cells Undergoing Apoptosis: Role of Apoptotic Blebs in Monocyte Chemotaxis Blood, August 1, 1999; 94(3): 1012 - 1020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. P. Mgbonyebi, J. Russo, and I. H. Russo Roscovitine Induces Cell Death and Morphological Changes Indicative of Apoptosis in MDA-MB-231 Breast Cancer Cells Cancer Res., April 1, 1999; 59(8): 1903 - 1910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Sgorbissa, R Benetti, S Marzinotto, C Schneider, and C Brancolini Caspase-3 and caspase-7 but not caspase-6 cleave Gas2 in vitro: implications for microfilament reorganization during apoptosis J. Cell Sci., January 12, 1999; 112(23): 4475 - 4482. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Ghibelli, C. Fanelli, G. Rotilio, E. Lafavia, S. Coppola, C. Colussi, P. Civitareale, and M. R. Ciriolo Rescue of cells from apoptosis by inhibition of active GSH extrusion FASEB J, April 1, 1998; 12(6): 479 - 486. [Abstract] [Full Text]
|
|
|
|
|
|
J. C. Mills, N. L. Stone, J. Erhardt, and R. N. Pittman Apoptotic Membrane Blebbing Is Regulated by Myosin Light Chain Phosphorylation J. Cell Biol., February 9, 1998; 140(3): 627 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Torgerson and M. McNiven The actin-myosin cytoskeleton mediates reversible agonist-induced membrane blebbing J. Cell Sci., January 10, 1998; 111(19): 2911 - 2922. [Abstract] [PDF]
|
|
|
|
 |
|
 K. McCall and H. Steller Requirement for DCP-1 Caspase During Drosophila Oogenesis Science, January 9, 1998; 279(5348): 230 - 234. [Abstract] [Full Text]
|
|
|
|
|
|
C. Brancolini, D. Lazarevic, J. Rodriguez, and C. Schneider Dismantling Cell-Cell Contacts during Apoptosis Is Coupled to a Caspase-dependent Proteolytic Cleavage of {beta}-Catenin J. Cell Biol., November 3, 1997; 139(3): 759 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Bratton, V. A. Fadok, D. A. Richter, J. M. Kailey, L. A. Guthrie, and P. M. Henson Appearance of Phosphatidylserine on Apoptotic Cells Requires Calcium-mediated Nonspecific Flip-Flop and Is Enhanced by Loss of the Aminophospholipid Translocase J. Biol. Chem., October 17, 1997; 272(42): 26159 - 26165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Nemes Jr., R. Adany, M. Balazs, P. Boross, and L. Fesus Identification of Cytoplasmic Actin as an Abundant Glutaminyl Substrate for Tissue Transglutaminase in HL-60 and U937 Cells Undergoing Apoptosis J. Biol. Chem., August 15, 1997; 272(33): 20577 - 20583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lindner, E. Holler, B. Ertl, G. Multhoff, M. Schreglmann, I. Klauke, S. Schultz-Hector, and G. Eissner Peripheral Blood Mononuclear Cells Induce Programmed Cell Death in Human Endothelial Cells and May Prevent Repair: Role of Cytokines Blood, March 15, 1997; 89(6): 1931 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I Guenal, Y Risler, and B Mignotte Down-regulation of actin genes precedes microfilament network disruption and actin cleavage during p53-mediated apoptosis J. Cell Sci., January 2, 1997; 110(4): 489 - 495. [Abstract] [PDF]
|
|
|
|
|
|
D. M. Vanags, M. I. Porn-Ares, S. Coppola, D. H. Burgess, and S. Orrenius Protease Involvement in Fodrin Cleavage and Phosphatidylserine Exposure in Apoptosis J. Biol. Chem., December 6, 1996; 271(49): 31075 - 31085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. S. Yoon, J. W. Kim, K. W. Kang, Y. S. Kim, K. H. Choi, and C. O Joe Poly(ADP-ribosyl)ation of Histone H1 Correlates with Internucleosomal DNA Fragmentation during Apoptosis J. Biol. Chem., April 12, 1996; 271(15): 9129 - 9134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Martin, G. A. O'Brien, W. K. Nishioka, A. J. McGahon, A. Mahboubi, T. C. Saido, and D. R. Green Proteolysis of Fodrin (Non-erythroid Spectrin) during Apoptosis J. Biol. Chem., March 24, 1995; 270(12): 6425 - 6428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Shi, J. Glynn, L. Guilbert, T. Cotter, R. Bissonnette, and D. Green Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas Science, July 10, 1992; 257(5067): 212 - 214. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Vitale, C. Romagnani, A. Puccetti, D. Olive, R. Costello, L. Chiossone, A. Pitto, A. Bacigalupo, L. Moretta, and M. C. Mingari Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: Engagement of CD33 induces apoptosis of leukemic cells PNAS, May 8, 2001; 98(10): 5764 - 5769. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
