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Detection of Polycyclic Aromatic Hydrocarbon-DNA Adducts in White Blood Cells from Coke Oven Workers: Correlation with Job Categories¹

Department of Toxicology, National Institute of Occupational Health, P.O. Box 8149 Dep, 0033 Oslo 1, Norway [S.Ø., A.H.], and The Haddow Laboratoires, Institute for Cancer Research, Cotswold Road, Belmont, Sutton, Surrey SM2 5NG, Great Britain [D.H.P., A.H.]

Polycyclic aromatic hydrocarbon (PAH)-DNA adducts were quantitatively determined by ultrasensitive radioimmunoassay (USERIA) and ³²P postlabeling in 128 DNA samples from WBCs of 68 coke oven workers and a local control group of 13 workers. Forty-four samples had a detectable adduct level by USERIA, with a mean of 0.390 fmol adducts/µg DNA (12.9 adducts/10⁸ nucleotides) in the exposed group compared to a mean of 0.316 fmol adducts/µg DNA (10.4 adducts/10⁸ nucleotides) in the control group. The mean adduct level with ³²P postlabeling was 0.05 fmol/µg DNA (1.67 adducts/10{su8} nucleotides) for the exposed group and 0.046 fmol/µg DNA (1.54 adducts/10⁸) nucleotides for the control group.

Based on job description the workers were divided in 4 groups: control, low-, medium-, and high-exposure group. Both methods produced a positive correlation coefficient between estimated exposure and PAH-DNA adduct levels. The significance levels determined with Kendall rank correlation were P = 0.0145 for USERIA and P = 0.0594 for ³²P postlabeling. Adduct levels determined by ³²P postlabeling showed a correlation with tobacco smoking in the control group. No significant correlation between PAH-DNA adduct levels measured by USERIA and ³²P postlabeling was found. These results show that these methods recognize different parts of the complex exposures in a coke oven plant.

¹ This work was supported by North Atlantic Treaty Organization Grant 880679 and Grant 42.70.08 from the Nordic Council of Ministers.

² To whom requests for reprints should be addressed.

Received 8/28/91. Accepted 12/27/91.

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