This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hastings, A. Articles by Irie, R. F. Search for Related Content PubMed PubMed Citation Articles by Hastings, A. Articles by Irie, R. F.

Production and Characterization of a Murine/Human Chimeric Anti-Idiotype Antibody That Mimics Ganglioside¹

Department of Surgical Oncology, School of Medicine [A. H., S. K., R. E. S., R. F. I.] and Department of Microbiology and Molecular Genetics, The Molecular Biology Institute [A. H., S. L. M.], University of California, Los Angeles-Jonsson Comprehensive Cancer Center, The University of California, Los Angeles, California 90024

The V_L and V_H from a murine anti-idiotypic antibody that mimics ganglioside have been cloned, sequenced, and expressed as a chimeric mouse/human IgG1 antibody. The chimeric antibody retained a binding specificity indistinguishable from the original murine antibody. The V_H was a member of Vgam 3.8 family. The sequences are discussed in terms of ways in which proteins may mimic ganglioside epitopes.

¹ This study was supported by NIH/National Cancer Institute Grants CA16858, CA12582, CA42396, and CA30647.

² To whom requests for reprints should be addressed, at: Department of Surgical Oncology, School of Medicine, The University of California, Los Angeles, CA 90024.

Received 10/ 7/91. Accepted 1/20/92.