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Induction of Mutation of a Synthetic c-Ha-ras Gene Containing Hypoxanthine¹

Faculty of Pharmaceutical Sciences, Hokkaido University, Nishi-6, Kita-12, Kita-ku, Sapporo 060, Japan [H. K., H. M., H. K., E. O.], and Biological Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104, Japan [S. N.]

The second base of codon 61 of a synthetic c-Ha-ras gene was replaced with a hypoxanthine residue in a site-specific manner. Transfection of this gene into NIH3T3 cells by the calcium phosphate procedure resulted in increased focus formation. Total DNA was extracted from transformed cells, and the sequences of the inserted c-Ha-ras DNA were analyzed by the polymerase chain reaction-single-strand conformation polymorphism method. Mutations with A (or hypoxanthine) to G transition were detected exclusively. These results suggest that the synthetic c-Ha-ras gene can be used for investigations of mutageneses caused by DNA lesions.

¹ This work was supported, in part, by a Grant-in-Aid from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan.

² To whom requests for reprints should be addressed, at Faculty of Pharmaceutical Sciences, Hokkaido University, Nishi-6, Kita-12, Kita-ku, Sapporo 060, Japan.

Received 10/ 3/91. Accepted 1/24/92.

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