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Measurement of Gastrin and Transforming Growth Factor Messenger RNA Levels in Colonic Carcinoma Cell Lines by Quantitative Polymerase Chain Reaction

Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, P.O. Royal Melbourne Hospital, Victoria, 3050, Australia

A synthetic DNA template has been constructed that is suitable for the quantitation of mRNAs encoding gastrin, transforming growth factor (TGF), cholecystokinin, and the 78-kDa gastrin-binding protein. The template was used to measure levels of gastrin and TGF mRNA in 7 colonic and 2 gastric carcinoma cell lines by the polymerase chain reaction. All lines produced detectable gastrin and TGF mRNA with amounts varying between 2.1 and 540 molecules of gastrin mRNA/10³ cells and 1.1 and 28 molecules of TGF mRNA/10³ cells. These results are consistent with the hypothesis that both gastrin and TGF act as autocrine growth factors in colon carcinoma cell lines.

¹ To whom requests for reprints should be addressed.

Received 9/ 5/91. Accepted 2/11/92.

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