| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Stress Protein Group, Joint Center for Radiation Therapy, Dana-Farber Cancer Institute, Boston, Massachusetts 02115
When cells are exposed to heat shock, heavy metals, amino acid analogues, and other stresses, the heat shock transcription factor (HSF) is activated. The HSF then binds to the promoter of the heat shock genes, stimulating transcription of the heat shock proteins. Here, we demonstrate that exposure of NIH-3T3 cells to oxidants (H2O2 or menadione) also causes activation of the HSF. This activation is not blocked by inhibitors of protein synthesis (cycloheximide) or by inhibitors of protein kinases (2-aminopurine or genistein). In addition, the oxidant activated HSF is located in the nucleus of the cells. However, oxidant activation of the HSF does not result in the accumulation of hsp70 mRNA or of heat shock proteins. This is in contrast to the accumulation of heat shock proteins seen after heat shock activation of the HSF. This suggests that oxidant induced activation of HSF binding may have a function different from that of heat induced activation of HSF binding.
1 Supported by NIH Grants R29CA-44940 and RO1 47407.
2 To whom requests for reprints should be addressed, at Dana-Farber Cancer Institute, Rm. JF205, 44 Binney St., Boston MA 02115.
Received 10/28/92. Accepted 11/11/92.
This article has been cited by other articles:
|
|
 |
|
  J. L. Staib, J. C. Quindry, J. P. French, D. S. Criswell, and S. K. Powers Increased temperature, not cardiac load, activates heat shock transcription factor 1 and heat shock protein 72 expression in the heart Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R432 - R439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Morrison, M. Dinges, K. D. Singleton, K. Odoms, H. R. Wong, and P. E. Wischmeyer Glutamine's protection against cellular injury is dependent on heat shock factor-1 Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1625 - C1632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, H. He, L. Yu, H. H.-x. Xia, M. C. M. Lin, Q. Gu, M. Li, B. Zou, X. An, B. Jiang, et al. HSF1 Down-regulates XAF1 through Transcriptional Regulation J. Biol. Chem., February 3, 2006; 281(5): 2451 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Boellmann, T. Guettouche, Y. Guo, M. Fenna, L. Mnayer, and R. Voellmy DAXX interacts with heat shock factor 1 during stress activation and enhances its transcriptional activity PNAS, March 23, 2004; 101(12): 4100 - 4105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. O'Brien, M. L. Cunningham, B. T. Spear, and H. P. Glauert Peroxisome Proliferators Do Not Activate the Transcription Factors AP-1, Early Growth Response-1, or Heat Shock Factors 1 and 2 in Rats or Hamsters Toxicol. Sci., September 1, 2002; 69(1): 139 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Kregel Molecular Biology of Thermoregulation: Invited Review: Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance J Appl Physiol, May 1, 2002; 92(5): 2177 - 2186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Guo, T. Guettouche, M. Fenna, F. Boellmann, W. B. Pratt, D. O. Toft, D. F. Smith, and R. Voellmy Evidence for a Mechanism of Repression of Heat Shock Factor 1 Transcriptional Activity by a Multichaperone Complex J. Biol. Chem., November 30, 2001; 276(49): 45791 - 45799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chang, A. A. Knowlton, F. Xu, and J. S. Wasser Activation of the heat shock response: relationship to energy metabolites. A 31P NMR study in rat hearts Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H426 - H433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Adrie, C. Richter, M. Bachelet, N. Banzet, D. Francois, A. T. Dinh-Xuan, J. F. Dhainaut, B. S. Polla, and M.-J. Richard Contrasting effects of NO and peroxynitrites on HSP70 expression and apoptosis in human monocytes Am J Physiol Cell Physiol, August 1, 2000; 279(2): C452 - C460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Ruiz-Dueñas, F. Guillén, S. Camarero, M. Pérez-Boada, M. J. Martínez, and A. T. Martínez Regulation of Peroxidase Transcript Levels in Liquid Cultures of the Ligninolytic Fungus Pleurotus eryngii Appl. Envir. Microbiol., October 1, 1999; 65(10): 4458 - 4463. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Nishizawa, A. Nakai, K. Matsuda, M. Komeda, T. Ban, and K. Nagata Reactive Oxygen Species Play an Important Role in the Activation of Heat Shock Factor 1 in Ischemic-Reperfused Heart Circulation, February 23, 1999; 99(7): 934 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. J. Lee and P. M. Corry Metabolic Oxidative Stress-induced HSP70 Gene Expression Is Mediated through SAPK Pathway. ROLE OF Bcl-2 AND c-Jun NH2-TERMINAL KINASE J. Biol. Chem., November 6, 1998; 273(45): 29857 - 29863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yoshima, T. Yura, and H. Yanagi Heat Shock Factor 1 Mediates Hemin-induced hsp70 Gene Transcription in K562 Erythroleukemia Cells J. Biol. Chem., September 25, 1998; 273(39): 25466 - 25471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Chu, R. Zhong, F. Soncin, M. A. Stevenson, and S. K. Calderwood Transcriptional Activity of Heat Shock Factor 1 at 37 oC Is Repressed through Phosphorylation on Two Distinct Serine Residues by Glycogen Synthase Kinase 3alpha and Protein Kinases Calpha and Czeta J. Biol. Chem., July 17, 1998; 273(29): 18640 - 18646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Xia, Y. Guo, N. Vilaboa, J. Zuo, and R. Voellmy Transcriptional Activation of Heat Shock Factor HSF1 Probed by Phosphopeptide Analysis of Factor 32P-labeled in Vivo J. Biol. Chem., April 10, 1998; 273(15): 8749 - 8755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Michael, B. A. Markewitz, and D. E. Kohan Oxidant stress regulates basal endothelin-1 production by cultured rat pulmonary endothelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 1997; 273(4): L768 - L774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Holmberg, S. Leppa, J. E. Eriksson, and L. Sistonen The Phorbol Ester 12-O-Tetradecanoylphorbol 13-Acetate Enhances the Heat-induced Stress Response J. Biol. Chem., March 7, 1997; 272(10): 6792 - 6798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Xia and R. Voellmy Hyperphosphorylation of Heat Shock Transcription Factor 1Is Correlated with Transcriptional Competence and Slow Dissociation of Active Factor Trimers J. Biol. Chem., February 14, 1997; 272(7): 4094 - 4102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Kelly, P. M. Tiidus, M. E. Houston, and E. G. Noble Effect of vitamin E deprivation and exercise training on induction of HSP70 J Appl Physiol, December 1, 1996; 81(6): 2379 - 2385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Chu, F. Soncin, B. D. Price, M. A. Stevenson, and S. K. Calderwood Sequential Phosphorylation by Mitogen-activated Protein Kinase and Glycogen Synthase Kinase 3Represses Transcriptional Activation by Heat Shock Factor-1 J. Biol. Chem., November 29, 1996; 271(48): 30847 - 30857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nishizawa, A. Nakai, T. Higashi, M. Tanabe, S. Nomoto, K. Matsuda, T. Ban, and K. Nagata Reperfusion Causes Significant Activation of Heat Shock Transcription Factor 1 in Ischemic Rat Heart Circulation, November 1, 1996; 94(9): 2185 - 2192. [Abstract] [Full Text]
|
|
|
|
|
|
C. M. Cahill, W. R. Waterman, Y. Xie, P. E. Auron, and StuartK. Calderwood Transcriptional repression of the prointerleukin 1beta gene by heat shock factor 1 J. Biol. Chem., October 4, 1996; 271(40): 24874 - 24879. [Full Text]
|
|
|
|
|
|
H. Liu, R. Lightfoot, and J. L. Stevens Activation of Heat Shock Factor by Alkylating Agents Is Triggered by Glutathione Depletion and Oxidation of Protein Thiols J. Biol. Chem., March 1, 1996; 271(9): 4805 - 4812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X D Liu and D J Thiele Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription. Genes & Dev., March 1, 1996; 10(5): 592 - 603. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Liu, P. Corry, and Y. Lee Regulation of chemical stress-induced hsp70 gene expression in murine L929 cells J. Cell Sci., January 8, 1994; 107(8): 2209 - 2214. [Abstract] [PDF]
|
|
|
|
|
|
A. Gosslau, P. Ruoff, S. Mohsenzadeh, U. Hobohm, and L. Rensing Heat Shock and Oxidative Stress-induced Exposure of Hydrophobic Protein Domains as Common Signal in the Induction of hsp68 J. Biol. Chem., January 12, 2001; 276(3): 1814 - 1821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. F. Winklhofer, A. Reintjes, M. C. Hoener, R. Voellmy, and J. Tatzelt Geldanamycin Restores a Defective Heat Shock Response in Vivo J. Biol. Chem., November 21, 2001; 276(48): 45160 - 45167. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
