This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sandberg-Nordqvist, A.-C. Articles by Sara, V. Search for Related Content PubMed PubMed Citation Articles by Sandberg-Nordqvist, A.-C. Articles by Sara, V.

Characterization of Insulin-like Growth Factor 1 in Human Primary Brain Tumors¹

Department of Pathology [A-C. S-N., P-A. S., V. S.], Ludwig Institute of Cancer Research [V. P. C.], and Department of Neurosurgery [H. v. H.], Karolinska Hospital, Stockholm, Sweden; BioScience Center [P-A. S.], KaBI Pharmacia, Stockholm, Sweden; and Institute of Anatomy [M. R.], University of Zurich, Zurich, Switzerland

Insulin-like growth factor 1 (IGF-1) is involved in the regulation of brain development and has been suggested as an autocrine stimulator of brain tumor cell proliferation. This study demonstrates the expression of IGF-1 in tumor tissue from human gliomas and one esthesioneuroblastoma. Using immunohistochemistry, expression of an IGF-1-like peptide was localized in tumor cells of 6 of the 9 gliomas examined as well as the esthesioneuroblastoma. From one anaplastic oligodendroglioma (which showed strong IGF-1 immunostaining) the IGF-1 transcripts were characterized after isolation of mRNA followed by amplification using the reverse transcriptase-polymerase chain reaction. Two IGF-1 complementary DNAs resulting from alternative splicing of the IGF-1 primary transcript were identified. These transcripts encode two different precursor proteins which correspond to Ea IGF-1 and Eb IGF-1. The significance of IGF-1 alternative mRNA splicing pathways remains to be determined.

¹ Supported by the Swedish Medical Research Council, the Swedish Cancer Society, the Cancer Society in Stockholm, the Hans and Loo Osterman Foundation, and the Swiss National Foundation.

² To whom requests for reprints should be addressed, at Department of Pathology, Karolinska Hospital, Box 60500, S-104 01 Stockholm, Sweden.

Received 3/19/93. Accepted 4/22/93.

This article has been cited by other articles:

				A. A. Samani, S. Yakar, D. LeRoith, and P. Brodt The Role of the IGF System in Cancer Growth and Metastasis: Overview and Recent Insights Endocr. Rev., February 1, 2007; 28(1): 20 - 47. [Abstract] [Full Text] [PDF]

				W Zumkeller and M Westphal The IGF/IGFBP system in CNS malignancy Mol. Pathol., August 1, 2001; 54(4): 227 - 229. [Abstract] [Full Text] [PDF]

				L. Wang and M. L. Adamo Cyclic Adenosine 3',5'-Monophosphate Inhibits Insulin-Like Growth Factor I Gene Expression in Rat Glioma Cell Lines: Evidence for Regulation of Transcription and Messenger Ribonucleic Acid Stability Endocrinology, July 1, 2001; 142(7): 3041 - 3050. [Abstract] [Full Text] [PDF]

				H. M. Khandwala, I. E. McCutcheon, A. Flyvbjerg, and K. E. Friend The Effects of Insulin-Like Growth Factors on Tumorigenesis and Neoplastic Growth Endocr. Rev., June 1, 2000; 21(3): 215 - 244. [Abstract] [Full Text]

				G. N. Fuller, C. H. Rhee, K. R. Hess, L. S. Caskey, R. Wang, J. M. Bruner, W. K. A. Yung, and W. Zhang Reactivation of Insulin-like Growth Factor Binding Protein 2 Expression in Glioblastoma Multiforme: A Revelation by Parallel Gene Expression Profiling Cancer Res., September 1, 1999; 59(17): 4228 - 4232. [Abstract] [Full Text] [PDF]

				S. Sultana, R. Zhou, M. S. Sadagopan, and O. Skalli Effects of Growth Factors and Basement Membrane Proteins on the Phenotype of U-373 MG Glioblastoma Cells as Determined by the Expression of Intermediate Filament Proteins Am. J. Pathol., October 1, 1998; 153(4): 1157 - 1168. [Abstract] [Full Text] [PDF]